Reliable diagnosis of murine type 1 diabetes using a panel of autoantigens and "antigen surrogates" mounted onto a liquid array

Todd M. Doran, Jumpei Morimoto, Scott Simanski, Patrick J. McEnaney, Thomas Kodadek

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Autoantibodies raised against β cell antigens are the most reliable preclinical biomarkers for predicting the imminent onset of type 1 diabetes mellitus (T1DM). The most current detection platforms are technically challenging or are run on clinically esoteric equipment. Here, we present a straightforward approach to detect autoantibody biomarkers that employs highly PEGylated microspheres onto which are mounted various capture agents that include affinity-tagged antigens or small molecule "antigen surrogates." After incubation with small quantities of serum, the bound autoantibodies can be measured using a standard flow cytometer. By multiplexing this assay, we show that a panel of antigen and antigen surrogates reliably predicts hyperglycemia in a mouse model of diabetes without false positives.

Original languageEnglish (US)
Pages (from-to)3156-3163
Number of pages8
JournalMolecular BioSystems
Volume11
Issue number11
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© The Royal Society of Chemistry 2015.

Fingerprint Dive into the research topics of 'Reliable diagnosis of murine type 1 diabetes using a panel of autoantigens and "antigen surrogates" mounted onto a liquid array'. Together they form a unique fingerprint.

Cite this