Release of gonadotropin-releasing hormone from the Japanese quail hypothalamus in vitro

J. R. Millam, W. H. Burke, M E Elhalawani

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The regulation of GnRH (gonadotropin-releasing hormone) secretion in Japanese quail was studied by maintaining excised hypothalamic slices containing the median eminence region in a continuous flow superfusion system. GnRH released into the superfusate was measured by radioimmunoassay using an antibody generated against synthetic GnRH. GnRH secretion increased in a dose-response manner when hypothalamic slices from female quail were exposed to superfusion medium containing elevated potassium ion concentrations. The potassium-induced GnRH release was found to be calcium dependent. GnRH was also increased when either 1 × 10-6 M norepinephrine, epinephrine, or 1 × 10-5 M of the β-adrenergic agonist, isoproterenol was added to the superfusion medium. GnRH secretion from hypothalami of castrate and intact male quail was measured on Days 1, 10, and 21 of photostimulation and was elevated on Day 21. No differences were observed between the castrate and intact groups. It is concluded that GnRH is released from the quail hypothalamus in a manner analogous to mammals and that its release is subject to adrenergic stimulation.

Original languageEnglish (US)
Pages (from-to)293-301
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume53
Issue number2
DOIs
StatePublished - Jan 1 1984

Fingerprint

Coturnix
gonadotropin-releasing hormone
hypothalamus
Gonadotropin-Releasing Hormone
Hypothalamus
Quail
hormone secretion
quails
Potassium
potassium
adrenergic agonists
Adrenergic Agonists
Median Eminence
In Vitro Techniques
Coturnix japonica
epinephrine
norepinephrine
radioimmunoassays
Isoproterenol
Adrenergic Agents

Cite this

Release of gonadotropin-releasing hormone from the Japanese quail hypothalamus in vitro. / Millam, J. R.; Burke, W. H.; Elhalawani, M E.

In: General and Comparative Endocrinology, Vol. 53, No. 2, 01.01.1984, p. 293-301.

Research output: Contribution to journalArticle

Millam, J. R. ; Burke, W. H. ; Elhalawani, M E. / Release of gonadotropin-releasing hormone from the Japanese quail hypothalamus in vitro. In: General and Comparative Endocrinology. 1984 ; Vol. 53, No. 2. pp. 293-301.
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