Relayed magnetization transfer from nuclear Overhauser effect and chemical exchange observed by in vivo 31P MRS in rat brain

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Abstract

The 31P magnetization transfer effects among nuclear magnetic resonances (NMRs) of phosphocreatine (PCr), γ-adenosine-5'-triphosphate (γ-ATP) and inorganic phosphate (Pi) have been attributed to the chemical exchange reactions among PCr, ATP and Pi catalyzed by creatine kinase (CK) and ATPase enzymes and, therefore, are commonly applied in situ to measure chemical exchange fluxes involving two chemically coupled CK and ATPase reactions (i.e., PCr↔ATP↔Pi) by selectively saturating γ-ATP resonance. Besides the expected reductions in the Pi and PCr NMR signals upon saturating γ-ATP resonance, one particularly interesting phenomenon showing decreases in α-ATP and β-ATP signals was also observed. The underlying mechanism was investigated and identified via saturating NMR of β-ATP in the present study. The unique relayed magnetization transfer effects through spin diffusion were observed in the rat brain using in vivo 31P magnetic resonance spectroscopy.

Original languageEnglish (US)
Pages (from-to)716-721
Number of pages6
JournalMagnetic Resonance Imaging
Volume30
Issue number5
DOIs
StatePublished - Jun 1 2012

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Adenosinetriphosphate
Rats
Magnetization
Brain
Adenosine Triphosphate
Phosphocreatine
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Creatine Kinase
Adenosine Triphosphatases
Magnetic resonance spectroscopy
Adenosine Monophosphate
Phosphates
Enzymes
Fluxes

Keywords

  • Chemical exchange
  • In vivo P MRS
  • Magnetization transfer
  • Nuclear Overhauser effect (NOE)
  • Spin diffusion

Cite this

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title = "Relayed magnetization transfer from nuclear Overhauser effect and chemical exchange observed by in vivo 31P MRS in rat brain",
abstract = "The 31P magnetization transfer effects among nuclear magnetic resonances (NMRs) of phosphocreatine (PCr), γ-adenosine-5'-triphosphate (γ-ATP) and inorganic phosphate (Pi) have been attributed to the chemical exchange reactions among PCr, ATP and Pi catalyzed by creatine kinase (CK) and ATPase enzymes and, therefore, are commonly applied in situ to measure chemical exchange fluxes involving two chemically coupled CK and ATPase reactions (i.e., PCr↔ATP↔Pi) by selectively saturating γ-ATP resonance. Besides the expected reductions in the Pi and PCr NMR signals upon saturating γ-ATP resonance, one particularly interesting phenomenon showing decreases in α-ATP and β-ATP signals was also observed. The underlying mechanism was investigated and identified via saturating NMR of β-ATP in the present study. The unique relayed magnetization transfer effects through spin diffusion were observed in the rat brain using in vivo 31P magnetic resonance spectroscopy.",
keywords = "Chemical exchange, In vivo P MRS, Magnetization transfer, Nuclear Overhauser effect (NOE), Spin diffusion",
author = "Fei Du and Yi Zhang and Wei Chen",
year = "2012",
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language = "English (US)",
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pages = "716--721",
journal = "Magnetic Resonance Imaging",
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TY - JOUR

T1 - Relayed magnetization transfer from nuclear Overhauser effect and chemical exchange observed by in vivo 31P MRS in rat brain

AU - Du, Fei

AU - Zhang, Yi

AU - Chen, Wei

PY - 2012/6/1

Y1 - 2012/6/1

N2 - The 31P magnetization transfer effects among nuclear magnetic resonances (NMRs) of phosphocreatine (PCr), γ-adenosine-5'-triphosphate (γ-ATP) and inorganic phosphate (Pi) have been attributed to the chemical exchange reactions among PCr, ATP and Pi catalyzed by creatine kinase (CK) and ATPase enzymes and, therefore, are commonly applied in situ to measure chemical exchange fluxes involving two chemically coupled CK and ATPase reactions (i.e., PCr↔ATP↔Pi) by selectively saturating γ-ATP resonance. Besides the expected reductions in the Pi and PCr NMR signals upon saturating γ-ATP resonance, one particularly interesting phenomenon showing decreases in α-ATP and β-ATP signals was also observed. The underlying mechanism was investigated and identified via saturating NMR of β-ATP in the present study. The unique relayed magnetization transfer effects through spin diffusion were observed in the rat brain using in vivo 31P magnetic resonance spectroscopy.

AB - The 31P magnetization transfer effects among nuclear magnetic resonances (NMRs) of phosphocreatine (PCr), γ-adenosine-5'-triphosphate (γ-ATP) and inorganic phosphate (Pi) have been attributed to the chemical exchange reactions among PCr, ATP and Pi catalyzed by creatine kinase (CK) and ATPase enzymes and, therefore, are commonly applied in situ to measure chemical exchange fluxes involving two chemically coupled CK and ATPase reactions (i.e., PCr↔ATP↔Pi) by selectively saturating γ-ATP resonance. Besides the expected reductions in the Pi and PCr NMR signals upon saturating γ-ATP resonance, one particularly interesting phenomenon showing decreases in α-ATP and β-ATP signals was also observed. The underlying mechanism was investigated and identified via saturating NMR of β-ATP in the present study. The unique relayed magnetization transfer effects through spin diffusion were observed in the rat brain using in vivo 31P magnetic resonance spectroscopy.

KW - Chemical exchange

KW - In vivo P MRS

KW - Magnetization transfer

KW - Nuclear Overhauser effect (NOE)

KW - Spin diffusion

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