Relayed magnetization transfer from nuclear Overhauser effect and chemical exchange observed by in vivo 31P MRS in rat brain

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6 Scopus citations

Abstract

The 31P magnetization transfer effects among nuclear magnetic resonances (NMRs) of phosphocreatine (PCr), γ-adenosine-5'-triphosphate (γ-ATP) and inorganic phosphate (Pi) have been attributed to the chemical exchange reactions among PCr, ATP and Pi catalyzed by creatine kinase (CK) and ATPase enzymes and, therefore, are commonly applied in situ to measure chemical exchange fluxes involving two chemically coupled CK and ATPase reactions (i.e., PCr↔ATP↔Pi) by selectively saturating γ-ATP resonance. Besides the expected reductions in the Pi and PCr NMR signals upon saturating γ-ATP resonance, one particularly interesting phenomenon showing decreases in α-ATP and β-ATP signals was also observed. The underlying mechanism was investigated and identified via saturating NMR of β-ATP in the present study. The unique relayed magnetization transfer effects through spin diffusion were observed in the rat brain using in vivo 31P magnetic resonance spectroscopy.

Original languageEnglish (US)
Pages (from-to)716-721
Number of pages6
JournalMagnetic Resonance Imaging
Volume30
Issue number5
DOIs
StatePublished - Jun 1 2012

Keywords

  • Chemical exchange
  • In vivo P MRS
  • Magnetization transfer
  • Nuclear Overhauser effect (NOE)
  • Spin diffusion

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