TY - JOUR
T1 - Relative expression of the products of glyoxylate bypass operon
T2 - Contributions of transcription and translation
AU - Chung, T.
AU - Resnik, E.
AU - Stueland, C.
AU - LaPorte, D. C.
PY - 1993
Y1 - 1993
N2 - Although the genes of the aceBAK operon are expressed from the same promoter, the relative cellular levels of their products are approximately 0.3:1:0.003. Gene and operon fusions with lacZ were constructed to characterize this differential expression. The upshift in expression between aceB and aceA resulted from differences in translational efficiency. In contrast, inefficient translation and premature transcriptional termination contributed to the downshift in expression between aceA and aceK. Premature transcriptional termination occurred within aceK and appears to result from inefficient translation. Deletion of repetitive extragenic palindromic elements between aceA and aceK had little effect on the relative expression of these genes. Rather, the sequences responsible for inefficient expression of aceK lie within the aceK ribosome binding site.
AB - Although the genes of the aceBAK operon are expressed from the same promoter, the relative cellular levels of their products are approximately 0.3:1:0.003. Gene and operon fusions with lacZ were constructed to characterize this differential expression. The upshift in expression between aceB and aceA resulted from differences in translational efficiency. In contrast, inefficient translation and premature transcriptional termination contributed to the downshift in expression between aceA and aceK. Premature transcriptional termination occurred within aceK and appears to result from inefficient translation. Deletion of repetitive extragenic palindromic elements between aceA and aceK had little effect on the relative expression of these genes. Rather, the sequences responsible for inefficient expression of aceK lie within the aceK ribosome binding site.
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U2 - 10.1128/jb.175.14.4572-4575.1993
DO - 10.1128/jb.175.14.4572-4575.1993
M3 - Comment/debate
C2 - 8331088
AN - SCOPUS:0027227350
SN - 0021-9193
VL - 175
SP - 4572
EP - 4575
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 14
ER -