Relationships among fecal, air, oral, and tracheal microbial communities in pigs in a respiratory infection disease model

Robert Valeris-Chacin, Amanda Sponheim, Eduardo Fano, Richard Isaacson, Randall S. Singer, Joel Nerem, Fernando L. Leite, Maria Pieters

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The association of the lower respiratory tract microbiome in pigs with that of other tissues and environment is still unclear. This study aimed to describe the microbiome of tracheal and oral fluids, air, and feces in the late stage of Mycoplasma hyopneumoniae infection in pigs, and assess the association between the tracheal microbiome and those from air, feces, and oral fluids. Tracheal fluids (n = 73), feces (n = 71), oropharyngeal fluids (n = 8), and air (n = 12) were collected in seeder pigs (inoculated with M. hyopneumoniae) and contact pigs (113 days post exposure to seeder pigs). After DNA extraction, the V4 region from 16S rRNA gene was sequenced and reads were processed using Divisive Amplicon Denoising Algorithm (DADA2). Clostridium and Streptococcus were among the top five genera identified in all sample types. Mycoplasma hyopneumoniae in tracheal fluids was associated with a reduction of diversity and increment of M. hyorhinis, Glaesserella parasuis, and Pasteurella multocida in tracheal fluids, as well as a reduction of Ruminiclostridium, Barnesiella, and Lactobacillus in feces. Air contributed in a greater proportion to bacteria in the trachea compared with feces and oral fluids. In conclusion, evidence suggests the existence of complex interactions between bacterial communities from distant and distinct niches.

Original languageEnglish (US)
Article number252
Pages (from-to)1-15
Number of pages15
JournalMicroorganisms
Volume9
Issue number2
DOIs
StatePublished - Feb 2021

Bibliographical note

Funding Information:
Funding: Financial support for this investigation was provided by Boehringer Ingelheim Animal Health USA Inc.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Environment
  • Fecal
  • Microbiome
  • Mycoplasma hyopneumoniae
  • Swine
  • Tracheal

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