Relationship of prostatic acid phosphatase localization in human prostate by a monoclonal antibody with the gleason grading system

Akhouri A Sinha, Donald F. Gleason, Michael J Wilson, Mark R. Wick, Pratap K. Reddy, Clyde E. Blackard

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Prostatic acid phosphatase (PAP) was localized in human prostate with a monoclonal antibody prepared against PAP isoenzyme II to determine patterns of its expression in normal, hyperplastic (BPH), and cancerous glands. The monoclonal antibody reacted with both isoenzymes II and IV in immunoblot studies. Formalin‐fixed, paraffin‐embedded tissue was used from patients who had not been treated with hormones or chemotherapy. In normal glands and BPH, there was marked variation in the intensity of PAP staining in morphologically otherwise similar epithelial cells. There was similar heterogeneity of staining in the adenocarcinomas. Rough quantification of the intensity patterns in the clinical groups indicated a slight shift to more intense staining in BPH and well‐differentiated carcinomas but a progressive decline in the PAP staining in the moderately and poorly differentiated tumors. This decrease in intracellular staining with decreasing differentiation is not inconsistent with the clinical observation that serum levels of acid phosphatase generally increase with higher grade and disseminated tumors, since the enzyme is simply more accessible to the circulatory system in those cases. The same decrease may explain the few disseminated tumors that are not associated with elevated serum levels.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalThe Prostate
Volume13
Issue number1
DOIs
StatePublished - 1988

Keywords

  • human prostate
  • immunohistochemistry
  • prostatic acid phosphatase

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