TY - JOUR
T1 - Relationship between antigen levels in middle ear and antigen passage through round window membrane in antigen-induced otitis media
AU - Hamaguchi, Y.
AU - Juhn, S. K.
AU - Morizono, T.
N1 - Funding Information:
This research was supported by NINCDS Grant NS-14538 for Otitis Media Pathogenesis Research Program.
PY - 1989
Y1 - 1989
N2 - By using an antigen-induced (Arthus) otitis media (OM) model in chinchillas sensitized with human serum albumin (HSA), we investigated the passage of HSA through the round window membrane (RWM) by measuring HSA levels in both the middle ear fluid (MEF) and the perilymph (PL). The effect of corticosteroid treatment on HSA passage was also studied. Mean HSA levels in both the MEF and the PL were proportional to the HSA dose administered (0.5, 5, 50 mg/ml). The proportion of HSA-positive PL samples was significantly lower in the sensitized group than that of the control (p<0.01). HSA levels in the PL appear to depend on HSA levels in the MEF, which were significantly depressed in the sensitized animals. These results suggest that an immunological reaction may be involved in the reduction of antigen (HSA) passage through the RWM by reducing antigen concentrations in the MEF. Corticosteroid treatment reduces the passage of HSA into the PL in non-sensitized animals.
AB - By using an antigen-induced (Arthus) otitis media (OM) model in chinchillas sensitized with human serum albumin (HSA), we investigated the passage of HSA through the round window membrane (RWM) by measuring HSA levels in both the middle ear fluid (MEF) and the perilymph (PL). The effect of corticosteroid treatment on HSA passage was also studied. Mean HSA levels in both the MEF and the PL were proportional to the HSA dose administered (0.5, 5, 50 mg/ml). The proportion of HSA-positive PL samples was significantly lower in the sensitized group than that of the control (p<0.01). HSA levels in the PL appear to depend on HSA levels in the MEF, which were significantly depressed in the sensitized animals. These results suggest that an immunological reaction may be involved in the reduction of antigen (HSA) passage through the RWM by reducing antigen concentrations in the MEF. Corticosteroid treatment reduces the passage of HSA into the PL in non-sensitized animals.
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U2 - 10.3109/00016488809138894
DO - 10.3109/00016488809138894
M3 - Article
C2 - 2522711
AN - SCOPUS:84907120200
SN - 0365-5237
VL - 107
SP - 124
EP - 128
JO - Acta Oto-Laryngologica, Supplement
JF - Acta Oto-Laryngologica, Supplement
IS - 457
ER -