Relation between plasma homocysteine concentration, the 844ins68 variant of the cystathionine β-synthase gene, and pyridoxal-5'-phosphate concentration

Michael Y. Tsai, Feng Yang, Michelle Bignell, Ömer Aras, Naomi Q. Hanson

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

A moderately elevated plasma total homocysteine (tHcy), whether measured during fasting or post-methionine load (PML), is recognized as a risk factor for coronary artery diseases (CAD). Cystathionine β-synthase (CBS), a key enzyme in the transsulfuration pathway, is important for the metabolism of homocysteine. In recent years, a relatively prevalent mutation, the 844ins68 (68-bp insertion), was found to be carried by about 12% of the general population. In the current investigation, we studied 741 individuals with respect to the effect of the 68-bp insertion of the CBS gene on fasting and PML tHcy, and also determined the level of pyridoxal-5'-phosphate (vitamin B6), a cofactor of the CBS enzyme. Our results showed that the mean fasting and PML increase in tHcy levels were lower in individuals carrying the 844ins68 variant compared to those without the insertion; although only the difference in PML increase in tHcy reached statistical significance (P = 0.02). When these individuals were divided into two groups based on vitamin B6 concentration, the PML increase in tHcy was significantly lower in individuals heterozygous for the insertion compared to those without the insertion only in the group of individuals whose vitamin B6 concentrations were below the sample median (38.0 nmol/L). We speculate that the 68-bp insertion is associated with somewhat higher levels of CBS enzyme activity, and that the effect of this becomes more pronounced in the presence of relatively low concentrations of pyridoxal-5'-phosphate, a cofactor of the CBS enzyme.

Original languageEnglish (US)
Pages (from-to)352-356
Number of pages5
JournalMolecular Genetics and Metabolism
Volume67
Issue number4
DOIs
StatePublished - Aug 1999

Bibliographical note

Funding Information:
This study was supported in part by a grant from the American Heart Association, Minnesota Affiliate.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Cystathionine β-synthase
  • Homocysteine
  • Methionine loading
  • Pyridoxal-5'- phosphate (vitamin B)

Fingerprint Dive into the research topics of 'Relation between plasma homocysteine concentration, the 844ins68 variant of the cystathionine β-synthase gene, and pyridoxal-5'-phosphate concentration'. Together they form a unique fingerprint.

Cite this