Regulatory T cells suppress T cell activation at the pathologic site of human visceral leishmaniasis

Ambak K. Rai, Chandreshwar P. Thakur, Amar Singh, Tulika Seth, Sandeep K. Srivastava, Pushpendra Singh, Dipendra K. Mitra

Research output: Contribution to journalArticlepeer-review

68 Scopus citations


Suppression of T cell response is thought to be involved in the pathogenesis of visceral leishmaniasis (VL). Regulatory T cell (Treg) mediated immune-suppression is reported in animal models of Leishmania infection. However, their precise role among human patients still requires pathologic validation. The present study is aimed at understanding the frequency dynamics and function of Treg cells in the blood and bone marrow (BM) of VL patients. The study included 42 parasitologically confirmed patients, 17 healthy contact and 9 normal bone marrow specimens (NBM). We show i) the selective accumulation of Treg cells at one of the disease inflicted site(s), the BM, ii) their in vitro expansion in response to LD antigen and iii) persistence after successful chemotherapy. Results indicate that the Treg cells isolated from BM produces IL-10 and may inhibit T cell activation in IL-10 dependent manner. Moreover, we observed significantly higher levels of IL-10 among drug unresponsive patients, suggesting their critical role in suppression of immunity among VL patients. Our results suggest that IL-10 plays an important role in suppression of host immunity in human VL and possibly determines the efficacy of chemotherapy.

Original languageEnglish (US)
Article numbere31551
JournalPloS one
Issue number2
StatePublished - Feb 8 2012


Dive into the research topics of 'Regulatory T cells suppress T cell activation at the pathologic site of human visceral leishmaniasis'. Together they form a unique fingerprint.

Cite this