Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration

Wenji Piao, Yanbao Xiong, Lushen Li, Vikas Saxena, Kile D. Smith, Keli L. Hippen, Christina Paluskievicz, Marina Willsonshirkey, Bruce R. Blazar, Reza Abdi, Jonathan S. Bromberg

Research output: Contribution to journalArticle


Regulatory T cells (Tregs) express high levels of cell surface lymphotoxin alpha beta (LTα1β2) to activate the LT beta receptor (LTβR) on the lymphatic endothelial cells (LECs), modulating LEC adhesion molecules, intercellular junctions, and chemokines. We demonstrate a role for Tregs through this pathway to condition the permissiveness of lymphatic endothelia for transendothelial migration (TEM), thus gating leukocyte traffic. Human Tregs share the same property with murine Tregs. Activation of TLR2 on Tregs during inflammation specifically augments LTα1β2-LTβR signaling, which further enhances the permissiveness of LECs to facilitate TEM. The conditioning of endothelia may promote the resolution of inflammation by directing leukocytes out of tissues to lymphatic vessels and draining lymph nodes (dLNs). Thus, Tregs interact with lymphatic endothelia under homeostasis and inflammation and dictate endothelial permissiveness and gating mechanisms for subsequent leukocyte migration through endothelial barriers.

Original languageEnglish (US)
Pages (from-to)1052-1062.e5
JournalCell reports
Issue number4
StatePublished - Jan 28 2020


  • Toll-like receptor 2
  • lymphatic endothelial cells
  • lymphotoxin
  • regulatory T cells
  • transendothelial migration

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration'. Together they form a unique fingerprint.

  • Cite this

    Piao, W., Xiong, Y., Li, L., Saxena, V., Smith, K. D., Hippen, K. L., Paluskievicz, C., Willsonshirkey, M., Blazar, B. R., Abdi, R., & Bromberg, J. S. (2020). Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration. Cell reports, 30(4), 1052-1062.e5.