Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein

Marta J. Gonzalez-Hernandez; James D. Cavalcoli; Maureen A. Sartor; Rafael Contreras-Galindo; Fan Meng; Manhong Dai; Derek Dube; Anjan K. Saha; Scott D. Gitlin; Gilbert S. Omenn; Mark H. Kaplan; David M. Markovitz

doi:10.1128/JVI.00556-14

Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein

Marta J. Gonzalez-Hernandez, James D. Cavalcoli, Maureen A. Sartor, Rafael Contreras-Galindo, Fan Meng, Manhong Dai, Derek Dube, Anjan K. Saha, Scott D. Gitlin, Gilbert S. Omenn, Mark H. Kaplan, David M. Markovitz

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Approximately 8% of the human genome is made up of endogenous retroviral sequences. As the HIV-1 Tat protein activates the overall expression of the human endogenous retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which of the 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated peripheral blood lymphocytes from a healthy donor showed that Tat significantly activates expression of 26 unique HERV-K (HML-2) proviruses, silences 12, and does not significantly alter the expression of the remaining proviruses. Quantitative reverse transcription-PCR validation of the sequencing data was performed on Tattreated PBLs of seven donors using provirus-specific primers and corroborated the results with a substantial degree of quantitative similarity.

Original language	English (US)
Pages (from-to)	8924-8935
Number of pages	12
Journal	Journal of virology
Volume	88
Issue number	16
DOIs	https://doi.org/10.1128/JVI.00556-14
State	Published - 2014
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1128/JVI.00556-14

Find It

Find It at U of M Libraries

Cite this

@article{c4082ceeb3f54083bd02d4089274d32d,

title = "Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein",

abstract = "Approximately 8% of the human genome is made up of endogenous retroviral sequences. As the HIV-1 Tat protein activates the overall expression of the human endogenous retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which of the 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated peripheral blood lymphocytes from a healthy donor showed that Tat significantly activates expression of 26 unique HERV-K (HML-2) proviruses, silences 12, and does not significantly alter the expression of the remaining proviruses. Quantitative reverse transcription-PCR validation of the sequencing data was performed on Tattreated PBLs of seven donors using provirus-specific primers and corroborated the results with a substantial degree of quantitative similarity.",

author = "Gonzalez-Hernandez, {Marta J.} and Cavalcoli, {James D.} and Sartor, {Maureen A.} and Rafael Contreras-Galindo and Fan Meng and Manhong Dai and Derek Dube and Saha, {Anjan K.} and Gitlin, {Scott D.} and Omenn, {Gilbert S.} and Kaplan, {Mark H.} and Markovitz, {David M.}",

year = "2014",

doi = "10.1128/JVI.00556-14",

language = "English (US)",

volume = "88",

pages = "8924--8935",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "16",

}

TY - JOUR

T1 - Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein

AU - Gonzalez-Hernandez, Marta J.

AU - Cavalcoli, James D.

AU - Sartor, Maureen A.

AU - Contreras-Galindo, Rafael

AU - Meng, Fan

AU - Dai, Manhong

AU - Dube, Derek

AU - Saha, Anjan K.

AU - Gitlin, Scott D.

AU - Omenn, Gilbert S.

AU - Kaplan, Mark H.

AU - Markovitz, David M.

PY - 2014

Y1 - 2014

N2 - Approximately 8% of the human genome is made up of endogenous retroviral sequences. As the HIV-1 Tat protein activates the overall expression of the human endogenous retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which of the 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated peripheral blood lymphocytes from a healthy donor showed that Tat significantly activates expression of 26 unique HERV-K (HML-2) proviruses, silences 12, and does not significantly alter the expression of the remaining proviruses. Quantitative reverse transcription-PCR validation of the sequencing data was performed on Tattreated PBLs of seven donors using provirus-specific primers and corroborated the results with a substantial degree of quantitative similarity.

AB - Approximately 8% of the human genome is made up of endogenous retroviral sequences. As the HIV-1 Tat protein activates the overall expression of the human endogenous retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which of the 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated peripheral blood lymphocytes from a healthy donor showed that Tat significantly activates expression of 26 unique HERV-K (HML-2) proviruses, silences 12, and does not significantly alter the expression of the remaining proviruses. Quantitative reverse transcription-PCR validation of the sequencing data was performed on Tattreated PBLs of seven donors using provirus-specific primers and corroborated the results with a substantial degree of quantitative similarity.

UR - http://www.scopus.com/inward/record.url?scp=84904871470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904871470&partnerID=8YFLogxK

U2 - 10.1128/JVI.00556-14

DO - 10.1128/JVI.00556-14

M3 - Article

C2 - 24872592

AN - SCOPUS:84904871470

VL - 88

SP - 8924

EP - 8935

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 16

ER -

Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein

Abstract

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this