Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein

Marta J. Gonzalez-Hernandez; James D. Cavalcoli; Maureen A. Sartor; Rafael Contreras-Galindo; Fan Meng; Manhong Dai; Derek Dube; Anjan K. Saha; Scott D. Gitlin; Gilbert S. Omenn; Mark H. Kaplan; David M. Markovitz

doi:10.1128/JVI.00556-14

Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein

Marta J. Gonzalez-Hernandez, James D. Cavalcoli, Maureen A. Sartor, Rafael Contreras-Galindo, Fan Meng, Manhong Dai, Derek Dube, Anjan K. Saha, Scott D. Gitlin, Gilbert S. Omenn, Mark H. Kaplan, David M. Markovitz

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Approximately 8% of the human genome is made up of endogenous retroviral sequences. As the HIV-1 Tat protein activates the overall expression of the human endogenous retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which of the 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated peripheral blood lymphocytes from a healthy donor showed that Tat significantly activates expression of 26 unique HERV-K (HML-2) proviruses, silences 12, and does not significantly alter the expression of the remaining proviruses. Quantitative reverse transcription-PCR validation of the sequencing data was performed on Tattreated PBLs of seven donors using provirus-specific primers and corroborated the results with a substantial degree of quantitative similarity.

Original language	English (US)
Pages (from-to)	8924-8935
Number of pages	12
Journal	Journal of virology
Volume	88
Issue number	16
DOIs	https://doi.org/10.1128/JVI.00556-14
State	Published - 2014
Externally published	Yes

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Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein. / Gonzalez-Hernandez, Marta J.; Cavalcoli, James D.; Sartor, Maureen A.; Contreras-Galindo, Rafael; Meng, Fan; Dai, Manhong; Dube, Derek; Saha, Anjan K.; Gitlin, Scott D.; Omenn, Gilbert S.; Kaplan, Mark H.; Markovitz, David M.

In: Journal of virology, Vol. 88, No. 16, 2014, p. 8924-8935.

Research output: Contribution to journal › Article › peer-review

Gonzalez-Hernandez, Marta J. ; Cavalcoli, James D. ; Sartor, Maureen A. ; Contreras-Galindo, Rafael ; Meng, Fan ; Dai, Manhong ; Dube, Derek ; Saha, Anjan K. ; Gitlin, Scott D. ; Omenn, Gilbert S. ; Kaplan, Mark H. ; Markovitz, David M. / Regulation of the human endogenous retrovirus K (HML-2) transcriptome by the HIV-1 tat protein. In: Journal of virology. 2014 ; Vol. 88, No. 16. pp. 8924-8935.

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abstract = "Approximately 8% of the human genome is made up of endogenous retroviral sequences. As the HIV-1 Tat protein activates the overall expression of the human endogenous retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which of the 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated peripheral blood lymphocytes from a healthy donor showed that Tat significantly activates expression of 26 unique HERV-K (HML-2) proviruses, silences 12, and does not significantly alter the expression of the remaining proviruses. Quantitative reverse transcription-PCR validation of the sequencing data was performed on Tattreated PBLs of seven donors using provirus-specific primers and corroborated the results with a substantial degree of quantitative similarity.",

author = "Gonzalez-Hernandez, {Marta J.} and Cavalcoli, {James D.} and Sartor, {Maureen A.} and Rafael Contreras-Galindo and Fan Meng and Manhong Dai and Derek Dube and Saha, {Anjan K.} and Gitlin, {Scott D.} and Omenn, {Gilbert S.} and Kaplan, {Mark H.} and Markovitz, {David M.}",

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AU - Meng, Fan

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