Regulation of stress granule dynamics by Grb7 and FAK signalling pathway

Nien Pei Tsai, Ping Chih Ho, Li-Na Wei

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Cells form stress granules (SGs) in response to environmental stresses, which constitute cytoplasmic domains where mRNAs are stored and translation is halted. Although several components are found in SGs, it is poorly understood as to how SGs are formed and dissolved. We identified growth factor receptor-bound protein 7 (Grb7), an RNA-binding, translational regulator, as an integral component of SGs, which directly interacts with Hu antigen R (HuR) and is required for cells to form SGs. When stress is terminated, Grb7 is hyperphosphorylated by focal adhesion kinase (FAK), loses its ability to directly interact with HuR and is dissociated from SG components, thereby disrupting SGs in recovering cells. Consistently, dominant-negative hypophospho mutants of FAK and Grb7 significantly attenuate SG disassembly during recovery. FAK activation followed by its phosphorylating Grb7 constitutes a cell-autonomous signalling pathway that regulates the disassembly of SGs and translational stimulation during recovery. This is the first reported pathway actively regulating the dynamics of SGs.

Original languageEnglish (US)
Pages (from-to)715-726
Number of pages12
JournalEMBO Journal
Volume27
Issue number5
DOIs
StatePublished - Mar 5 2008

Keywords

  • FAK
  • Grb7
  • Stress granules

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