Regulation of neuronal survival and morphology by the E3 ubiquitin ligase RNF157

A. Matz, S. J. Lee, N. Schwedhelm-Domeyer, D. Zanini, A. Holubowska, M. Kannan, M. Farnworth, O. Jahn, M. C. Göpfert, J. Stegmüller

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Neuronal health is essential for the long-term integrity of the brain. In this study, we characterized the novel E3 ubiquitin ligase ring finger protein 157 (RNF157), which displays a brain-dominant expression in mouse. RNF157 is a homolog of the E3 ligase mahogunin ring finger-1, which has been previously implicated in spongiform neurodegeneration. We identified RNF157 as a regulator of survival in cultured neurons and established that the ligase activity of RNF157 is crucial for this process. We also uncovered that independently of its ligase activity, RNF157 regulates dendrite growth and maintenance. We further identified the adaptor protein APBB1 (amyloid beta precursor protein-binding, family B, member 1 or Fe65) as an interactor and proteolytic substrate of RNF157 in the control of neuronal survival. Here, the nuclear localization of Fe65 together with its interaction partner RNA-binding protein SART3 (squamous cell carcinoma antigen recognized by T cells 3 or Tip110) is crucial to trigger apoptosis. In summary, we described that the E3 ligase RNF157 regulates important aspects of neuronal development.

Original languageEnglish (US)
Pages (from-to)626-642
Number of pages17
JournalCell Death and Differentiation
Volume22
Issue number4
DOIs
StatePublished - Apr 4 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Macmillan Publishers Limited All rights reserved.

Fingerprint

Dive into the research topics of 'Regulation of neuronal survival and morphology by the E3 ubiquitin ligase RNF157'. Together they form a unique fingerprint.

Cite this