Regulation of milk lipid formation and secretion in the mouse mammary gland

James L. McManaman, Carol A. Palmer, Steven Anderson, Kaylee Schwertfeger, Margaret C. Neville

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Cytosolic lipid droplets (CLDs), the immediate precursors of milk lipids in lactating animals, undergo cell-specific changes in their formation and intracellular distribution during mammary gland differentiation. Cell biological studies indicate that CLD formation in mammary epithelial cells is regulated in part by AKT-dependent increases in glucose uptake. Proteomic studies show that CLDs from lactating mammary epithelial cells possess a distinct protein composition enriched in molecules involved in their secretion and intracellular transport. CLD secretion is dependent on lactation and requires the purine catabolic enzyme xanthine oxidoreductase (XOR). Confocal immunofluorescence microscopy of XOR in lactating and nonlactating mammary glands and biochemical analysis of secreted CLDs link the secretion process to the formation of a stable tripartite complex between XOR, adipophilin (ADPH), and butyrophilin (Btn). Together these studies provide a molecular and cellular framework for understanding the process of milk lipid formation.

Original languageEnglish (US)
Pages (from-to)263-279
Number of pages17
JournalAdvances in Experimental Medicine and Biology
Volume554
StatePublished - Oct 12 2004

Fingerprint

Human Mammary Glands
Xanthine Dehydrogenase
Milk
Lipids
Breast
Epithelial Cells
Secretory Pathway
Fluorescence Microscopy
Lactation
Confocal Microscopy
Proteomics
Confocal microscopy
Lipid Droplets
Glucose
Animals
Enzymes
Molecules
Proteins
Chemical analysis

Cite this

Regulation of milk lipid formation and secretion in the mouse mammary gland. / McManaman, James L.; Palmer, Carol A.; Anderson, Steven; Schwertfeger, Kaylee; Neville, Margaret C.

In: Advances in Experimental Medicine and Biology, Vol. 554, 12.10.2004, p. 263-279.

Research output: Contribution to journalArticle

McManaman, James L. ; Palmer, Carol A. ; Anderson, Steven ; Schwertfeger, Kaylee ; Neville, Margaret C. / Regulation of milk lipid formation and secretion in the mouse mammary gland. In: Advances in Experimental Medicine and Biology. 2004 ; Vol. 554. pp. 263-279.
@article{9e5b1a6397dd489c901aed4448f2c509,
title = "Regulation of milk lipid formation and secretion in the mouse mammary gland",
abstract = "Cytosolic lipid droplets (CLDs), the immediate precursors of milk lipids in lactating animals, undergo cell-specific changes in their formation and intracellular distribution during mammary gland differentiation. Cell biological studies indicate that CLD formation in mammary epithelial cells is regulated in part by AKT-dependent increases in glucose uptake. Proteomic studies show that CLDs from lactating mammary epithelial cells possess a distinct protein composition enriched in molecules involved in their secretion and intracellular transport. CLD secretion is dependent on lactation and requires the purine catabolic enzyme xanthine oxidoreductase (XOR). Confocal immunofluorescence microscopy of XOR in lactating and nonlactating mammary glands and biochemical analysis of secreted CLDs link the secretion process to the formation of a stable tripartite complex between XOR, adipophilin (ADPH), and butyrophilin (Btn). Together these studies provide a molecular and cellular framework for understanding the process of milk lipid formation.",
author = "McManaman, {James L.} and Palmer, {Carol A.} and Steven Anderson and Kaylee Schwertfeger and Neville, {Margaret C.}",
year = "2004",
month = "10",
day = "12",
language = "English (US)",
volume = "554",
pages = "263--279",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
publisher = "Springer New York",

}

TY - JOUR

T1 - Regulation of milk lipid formation and secretion in the mouse mammary gland

AU - McManaman, James L.

AU - Palmer, Carol A.

AU - Anderson, Steven

AU - Schwertfeger, Kaylee

AU - Neville, Margaret C.

PY - 2004/10/12

Y1 - 2004/10/12

N2 - Cytosolic lipid droplets (CLDs), the immediate precursors of milk lipids in lactating animals, undergo cell-specific changes in their formation and intracellular distribution during mammary gland differentiation. Cell biological studies indicate that CLD formation in mammary epithelial cells is regulated in part by AKT-dependent increases in glucose uptake. Proteomic studies show that CLDs from lactating mammary epithelial cells possess a distinct protein composition enriched in molecules involved in their secretion and intracellular transport. CLD secretion is dependent on lactation and requires the purine catabolic enzyme xanthine oxidoreductase (XOR). Confocal immunofluorescence microscopy of XOR in lactating and nonlactating mammary glands and biochemical analysis of secreted CLDs link the secretion process to the formation of a stable tripartite complex between XOR, adipophilin (ADPH), and butyrophilin (Btn). Together these studies provide a molecular and cellular framework for understanding the process of milk lipid formation.

AB - Cytosolic lipid droplets (CLDs), the immediate precursors of milk lipids in lactating animals, undergo cell-specific changes in their formation and intracellular distribution during mammary gland differentiation. Cell biological studies indicate that CLD formation in mammary epithelial cells is regulated in part by AKT-dependent increases in glucose uptake. Proteomic studies show that CLDs from lactating mammary epithelial cells possess a distinct protein composition enriched in molecules involved in their secretion and intracellular transport. CLD secretion is dependent on lactation and requires the purine catabolic enzyme xanthine oxidoreductase (XOR). Confocal immunofluorescence microscopy of XOR in lactating and nonlactating mammary glands and biochemical analysis of secreted CLDs link the secretion process to the formation of a stable tripartite complex between XOR, adipophilin (ADPH), and butyrophilin (Btn). Together these studies provide a molecular and cellular framework for understanding the process of milk lipid formation.

UR - http://www.scopus.com/inward/record.url?scp=4644269107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644269107&partnerID=8YFLogxK

M3 - Article

VL - 554

SP - 263

EP - 279

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -