Regulation of Metabolic Homeostasis in Cell Culture Bioprocesses

Conor M. O'Brien, Bhanu Chandra Mulukutla, Douglas G. Mashek, Wei Shou Hu

Research output: Contribution to journalReview article

Abstract

Mammalian cells are the main tool for the production of therapeutic proteins, viruses for gene therapy, and cells for cell therapy. In production processes cell metabolism is the main driver that causes changes in the growth environment and affects productivity and product quality. Of all nutrients, glucose has the most prominent impact on bioprocesses. We summarize recent findings on the regulation of glucose and energy metabolism in cultured cells. Local allosteric regulations and post-translational modifications of enzymes in metabolic networks interplay with global signaling and transcriptional regulation. These regulatory networks sustain homeostasis across the cytosolic and mitochondrial compartments. Understanding the regulation of glucose metabolism and metabolic state is crucial for enhancing process productivity and product quality.

Original languageEnglish (US)
Pages (from-to)1113-1127
Number of pages15
JournalTrends in biotechnology
Volume38
Issue number10
DOIs
StatePublished - Oct 2020

Keywords

  • bioprocessing
  • energy metabolism
  • homeostasis

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