Regulation of HLA class I transcription in T cells

K. R. Zachow, H. T. Orr

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cortical thymocytes express a very low level of HLA class I Ag on their cell surface. As these cells mature to medullary thymocytes and peripheral T cells, HLA class I expression increases to a high level. Leukemic T cell lines arrested at various stages of development were used to examine the regulation of HLA class I expression. Nuclear runoff analysis demonstrated that expression differences in these cell lines were the result of a difference in transcription rates. Electrophoretic mobility shift analysis with the upstream regulatory region of HLA-A2 as a probe for HLA class I-specific DNA binding proteins indicated that DNA binding activity in the cell extracts paralleled the transcriptional activity of the cells. The predominant DNA binding activity in these T cell lines was mapped, by DNase I protection analysis, to a conserved inverted repeat of the MHC class I enhancer. Thus HLA class I expression in these stage-specific T cell lines correlates with the amount of this enhancer binding activity in these cells suggesting that this commonly found DNA binding factor may, in this system, act to regulate HLA class I expression in a very specific manner.

Original languageEnglish (US)
Pages (from-to)3385-3389
Number of pages5
JournalJournal of Immunology
Volume143
Issue number10
StatePublished - Jan 1 1989

Fingerprint

T-Lymphocytes
Cell Line
Thymocytes
DNA
HLA-A2 Antigen
Nucleic Acid Regulatory Sequences
Deoxyribonuclease I
DNA-Binding Proteins
Cell Extracts

Cite this

Regulation of HLA class I transcription in T cells. / Zachow, K. R.; Orr, H. T.

In: Journal of Immunology, Vol. 143, No. 10, 01.01.1989, p. 3385-3389.

Research output: Contribution to journalArticle

Zachow, KR & Orr, HT 1989, 'Regulation of HLA class I transcription in T cells', Journal of Immunology, vol. 143, no. 10, pp. 3385-3389.
Zachow, K. R. ; Orr, H. T. / Regulation of HLA class I transcription in T cells. In: Journal of Immunology. 1989 ; Vol. 143, No. 10. pp. 3385-3389.
@article{697c391b8a4e49ddac394c7722f95ef2,
title = "Regulation of HLA class I transcription in T cells",
abstract = "Cortical thymocytes express a very low level of HLA class I Ag on their cell surface. As these cells mature to medullary thymocytes and peripheral T cells, HLA class I expression increases to a high level. Leukemic T cell lines arrested at various stages of development were used to examine the regulation of HLA class I expression. Nuclear runoff analysis demonstrated that expression differences in these cell lines were the result of a difference in transcription rates. Electrophoretic mobility shift analysis with the upstream regulatory region of HLA-A2 as a probe for HLA class I-specific DNA binding proteins indicated that DNA binding activity in the cell extracts paralleled the transcriptional activity of the cells. The predominant DNA binding activity in these T cell lines was mapped, by DNase I protection analysis, to a conserved inverted repeat of the MHC class I enhancer. Thus HLA class I expression in these stage-specific T cell lines correlates with the amount of this enhancer binding activity in these cells suggesting that this commonly found DNA binding factor may, in this system, act to regulate HLA class I expression in a very specific manner.",
author = "Zachow, {K. R.} and Orr, {H. T.}",
year = "1989",
month = "1",
day = "1",
language = "English (US)",
volume = "143",
pages = "3385--3389",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "10",

}

TY - JOUR

T1 - Regulation of HLA class I transcription in T cells

AU - Zachow, K. R.

AU - Orr, H. T.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Cortical thymocytes express a very low level of HLA class I Ag on their cell surface. As these cells mature to medullary thymocytes and peripheral T cells, HLA class I expression increases to a high level. Leukemic T cell lines arrested at various stages of development were used to examine the regulation of HLA class I expression. Nuclear runoff analysis demonstrated that expression differences in these cell lines were the result of a difference in transcription rates. Electrophoretic mobility shift analysis with the upstream regulatory region of HLA-A2 as a probe for HLA class I-specific DNA binding proteins indicated that DNA binding activity in the cell extracts paralleled the transcriptional activity of the cells. The predominant DNA binding activity in these T cell lines was mapped, by DNase I protection analysis, to a conserved inverted repeat of the MHC class I enhancer. Thus HLA class I expression in these stage-specific T cell lines correlates with the amount of this enhancer binding activity in these cells suggesting that this commonly found DNA binding factor may, in this system, act to regulate HLA class I expression in a very specific manner.

AB - Cortical thymocytes express a very low level of HLA class I Ag on their cell surface. As these cells mature to medullary thymocytes and peripheral T cells, HLA class I expression increases to a high level. Leukemic T cell lines arrested at various stages of development were used to examine the regulation of HLA class I expression. Nuclear runoff analysis demonstrated that expression differences in these cell lines were the result of a difference in transcription rates. Electrophoretic mobility shift analysis with the upstream regulatory region of HLA-A2 as a probe for HLA class I-specific DNA binding proteins indicated that DNA binding activity in the cell extracts paralleled the transcriptional activity of the cells. The predominant DNA binding activity in these T cell lines was mapped, by DNase I protection analysis, to a conserved inverted repeat of the MHC class I enhancer. Thus HLA class I expression in these stage-specific T cell lines correlates with the amount of this enhancer binding activity in these cells suggesting that this commonly found DNA binding factor may, in this system, act to regulate HLA class I expression in a very specific manner.

UR - http://www.scopus.com/inward/record.url?scp=0024346902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024346902&partnerID=8YFLogxK

M3 - Article

C2 - 2809207

AN - SCOPUS:0024346902

VL - 143

SP - 3385

EP - 3389

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 10

ER -