Regulation of eosinophil recruitment and allergic airway inflammation by heparan sulfate proteoglycan (HSPG) modifying enzymes

Xiao Na Ge, Idil Bastan, Sung Gil Ha, Yana G. Greenberg, Jeffrey D. Esko, Savita P. Rao, P. Sriramarao

Research output: Contribution to journalArticle

Abstract

Background: HSPGs are glycoproteins containing covalently attached heparan sulfate (HS) chains which bind to growth factors, chemokines, etc., and regulate various aspects of inflammation including cell recruitment. We previously showed that deletion of endothelial N-acetylglucosamine N-deacetylase-N-sulfotransferase-1 (Ndst1), an enzyme responsible for N-sulfation during HS biosynthesis, reduces allergic airway inflammation (AAI). Here, we investigated the importance of O-sulfation mediated by uronyl 2-O-sulfotransferase (Hs2st) in development of AAI relative to N-sulfation. Methods: Mice deficient in endothelial and leukocyte Hs2st (Hs2st f/f Tie2Cre +) or Ndst1 (Ndst1 f/f Tie2Cre +) and WT mice were challenged with Alternaria alternata and evaluated for airway inflammation. Trafficking of murine eosinophils on lung endothelial cells was examined in vitro under conditions of flow. Results: Exposure to Alternaria decreased expression level of Hs2st in WT mice while level of Ndst1 remained unchanged. Compared to WT mice, Alternaria-challenged Hs2st f/f Tie2Cre + mice exhibited significantly increased eosinophils in the bone marrow, bronchoalveolar lavage fluid [BALF] and lung tissue associated with persistent airway hyperresponsiveness, airway mucus hypersecretion and elevated Th2 cytokines. In contrast, Alternaria-challenged Ndst1 f/f Tie2Cre + mice exhibited a marked reduction in airway eosinophilia, mucus secretion and smooth muscle mass compared to WT counterparts. While BALF eotaxins were lower in Alternaria-challenged Hs2st f/f Tie2Cre + relative to WT mice, they were not reduced to background levels as in allergen-challenged Ndst1 f/f Tie2Cre + mice. Trafficking of murine eosinophils under conditions of flow in vitro was similar on Hs2st-deficient and WT endothelial cells. Expression of ZO-1 in Hs2st-deficient lung blood vessels in control and allergen-challenged mice was significantly lower than in WT counterparts. Conclusions: Our study demonstrates that allergen exposure reduces expression of Hs2st; loss of uronyl 2-O-sulfation in endothelial and leukocyte HSPG amplifies recruitment of eosinophils likely due to a compromised vascular endothelium resulting in persistent inflammation whereas loss of N-sulfation limits eosinophilia and attenuates inflammation underscoring the importance of site-specific sulfation in HSPG to their role in AAI.

Original languageEnglish (US)
Pages (from-to)98-112
Number of pages15
JournalExperimental Lung Research
Volume44
Issue number2
DOIs
StatePublished - Feb 7 2018

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Sulfotransferases
Heparan Sulfate Proteoglycans
Eosinophils
Alternaria
Inflammation
Enzymes
Allergens
N-acetylglucosamine deacetylase
Heparitin Sulfate
Endothelial cells
Bronchoalveolar Lavage Fluid
Eosinophilia
Mucus
Lung
Leukocytes
Endothelial Cells
Fluids
Biosynthesis
Blood vessels
Chemokines

Keywords

  • Hs2st
  • Ndst1
  • allergic asthma
  • endothelial barrier
  • eosinophilia
  • trafficking

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

Cite this

Regulation of eosinophil recruitment and allergic airway inflammation by heparan sulfate proteoglycan (HSPG) modifying enzymes. / Ge, Xiao Na; Bastan, Idil; Ha, Sung Gil; Greenberg, Yana G.; Esko, Jeffrey D.; Rao, Savita P.; Sriramarao, P.

In: Experimental Lung Research, Vol. 44, No. 2, 07.02.2018, p. 98-112.

Research output: Contribution to journalArticle

Ge, Xiao Na ; Bastan, Idil ; Ha, Sung Gil ; Greenberg, Yana G. ; Esko, Jeffrey D. ; Rao, Savita P. ; Sriramarao, P. / Regulation of eosinophil recruitment and allergic airway inflammation by heparan sulfate proteoglycan (HSPG) modifying enzymes. In: Experimental Lung Research. 2018 ; Vol. 44, No. 2. pp. 98-112.
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AU - Ha, Sung Gil

AU - Greenberg, Yana G.

AU - Esko, Jeffrey D.

AU - Rao, Savita P.

AU - Sriramarao, P.

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