Abstract
T lymphocytes bearing γ/δ TCRs are a major population of T cells in neonatal calves and discrete subsets of γ/δ T cells display tissue-specific accumulation and responsiveness to infection. To enhance our understanding of the immunobiology of γ/δ T cells, we characterized the gene expression profile of circulating bovine γ/δ T cells following stimulation with recombinant human IL-2 and ConA. Statistical analysis of microarray data identified 108 genes with significantly altered expression, including four genes associated with apoptosis. Real-time reverse transcription-PCR (RT-PCR) analysis of 15 genes related to apoptotic pathways showed that both the Fas-mediated and the mitochondrial apoptotic pathways were repressed in circulating bovine γ/δ T cells in response to mitogen activation, indicating that stimulated peripheral bovine γ/δ T cells are resistant to activation-induced apoptosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 15-23 |
| Number of pages | 9 |
| Journal | Veterinary immunology and immunopathology |
| Volume | 105 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - May 1 2005 |
Bibliographical note
Funding Information:This work was supported in part by grants 99-35205-8618 and 00-52100-9612 from the United States Department of Agriculture.
Keywords
- Apoptosis
- Microarray
- Mitogenic stimulation
- Real-time PCR
- γ/δ T cell
