Regulation of antimicrobial peptide expression in human gingival keratinocytes by interleukin-1α

Yuka Hiroshima, Mika Bando, Masatoshi Kataoka, Yuji Inagaki, Mark C. Herzberg, Karen F. Ross, Kazuo Hosoi, Toshihiko Nagata, Jun Ichi Kido

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In the oral cavity, mucosal keratinocytes resist bacterial infection, in part, by producing broad-spectrum antimicrobial peptides (AMPs) including defensin, adrenomedullin and calprotectin. Epidermal keratinocyte expression of many AMPs increases in response to interleukin-1α (IL-1α). IL-1α is produced by epidermal keratinocytes and regulates cell differentiation. To better understand innate immunity in the oral cavity, we sought to determine how IL-1α might regulate expression of AMPs by human gingival keratinocytes (HGKs) using DNA microarray and Western blot analyses. HGKs from three subjects expressed eleven AMPs, including S100A7, S100A8, S100A9, S100A12, secretory leucocyte protease inhibitor, lipocalin 2 (LCN2), cystatin C and β-defensin 2. Of the expressed AMPs, S100A7, S100A12 and LCN2 were up-regulated by IL-1α (inducible AMPs); the other AMPs were considered to be constitutive. Human gingival keratinocytes, therefore, express constitutive and IL-1α-inducible AMPs to provide a rapid and robust innate response to microbial infection.

Original languageEnglish (US)
Pages (from-to)761-767
Number of pages7
JournalArchives of Oral Biology
Volume56
Issue number8
DOIs
StatePublished - Aug 1 2011

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Interleukin-1
Keratinocytes
Peptides
Defensins
Mouth
Secretory Leukocyte Peptidase Inhibitor
Leukocyte L1 Antigen Complex
Adrenomedullin
Cystatin C
Oligonucleotide Array Sequence Analysis
Bacterial Infections
Innate Immunity
Cell Differentiation
Western Blotting
Infection

Keywords

  • Antimicrobial peptide
  • Gingival keratinocyte
  • Interleukin-1α
  • Microarray analysis

Cite this

Regulation of antimicrobial peptide expression in human gingival keratinocytes by interleukin-1α. / Hiroshima, Yuka; Bando, Mika; Kataoka, Masatoshi; Inagaki, Yuji; Herzberg, Mark C.; Ross, Karen F.; Hosoi, Kazuo; Nagata, Toshihiko; Kido, Jun Ichi.

In: Archives of Oral Biology, Vol. 56, No. 8, 01.08.2011, p. 761-767.

Research output: Contribution to journalArticle

Hiroshima, Yuka ; Bando, Mika ; Kataoka, Masatoshi ; Inagaki, Yuji ; Herzberg, Mark C. ; Ross, Karen F. ; Hosoi, Kazuo ; Nagata, Toshihiko ; Kido, Jun Ichi. / Regulation of antimicrobial peptide expression in human gingival keratinocytes by interleukin-1α. In: Archives of Oral Biology. 2011 ; Vol. 56, No. 8. pp. 761-767.
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