A mapping population was developed from the two hard red spring wheat (Triticum aestivum L.) breeding lines MN98550-5 (PI 660540 MAP) and MN99394-1 (PI 660541 MAP) at the University of Minnesota. This population, MN98550-5/MN99394-1 (Reg. No. MP-4, NSL 472873 MAP), comprises 139 F 6:8 recombinant inbred lines (RILs). The primary objective of developing this mapping population was to identify the chromosome regions that influence end-use quality traits in hard red spring wheat lines that are adapted to the Upper Midwest region of the USA. The population was genotyped with diversity array technology and simple sequence repeat markers, and the genetic map was composed of 531 marker loci covering all 21 chromosomes of wheat. Forty-three quantitative trait loci (QTL) influenced dough-mixing strength and bread-making properties. This population also segregated for several important traits, including resistance to fungal diseases (leaf rust, caused by Puccinia triticina f. sp. tritici; and stem rust, caused by P. graminis f. sp. tritici), kernel characteristics, and agronomic traits, and their QTL and closely linked markers were identified. The information obtained from QTL analysis was useful in selecting the best RILs as parents in our breeding program.