Conventional cultivars with high yield, good seed quality and disease resistance are required for organic farming, soy food production, and some specific markets. Soybean [Glycine max (L.) Merr.] 'Brookings' (Reg. No. CV-512, PI 667735) was developed at South Dakota State University (SDSU), Brookings, SD, and released by the South Dakota Agricultural Experiment Station in 2012, for its high yield potential, good seed quality, and resistance to Phytophthora root rot, as well as adaptability to South Dakota and similar regions. Brookings (experimental line SD05-240) is a maturity group (MG) I cultivar (relative maturity 1.7). It originated from the F5 progeny of a single F4 plant derived from the cross of A00-711063 × SD98- 595 by a single-pod descent method. Following yield trials within the SDSU soybean breeding program, it was further evaluated for yield and seed quality traits through the USDA Northern Regional Uniform Soybean Preliminary Tests (UPT) in 2008, Uniform Soybean Tests (UT) in 2009 and 2010, and the South Dakota Crop Performance Tests (CPT) in 2009 and 2010. The yield of Brookings in the SD CPT averaged 4257 kg ha-1, 14.2% higher than the check cultivar Deuel and 8.9% higher than 'MN1410' (P < 0.05). In the MG II UPT (11 locations) and MG I UT tests (29 environments), Brookings exhibited a mean yield comparable to check cultivars IA1022 and MN1410. The average protein concentration of Brookings was higher than IA1022 but lower than MN1410, and its oil content was similar to MN1410 but lower than IA1022. It had the best seed quality among the test entries. In addition, Brookings has the Rps1k allele for resistance to Phytophthora sojae, which is derived from the parent SD98-595, and exhibited resistance to races 4 and 7 of P. sojae. Brookings is a conventional MG I cultivar and thus a good option for producers of non-genetically modified soybeans in South Dakota and similar regions.