Refined structures of three crystal forms of toxic shock syndrome toxin- 1 and of a tetramutant with reduced activity

G. Sridhar Prasad, Ramaswamy Radhakrishnan, David T. Mitchell, Cathleen A. Earhart, Martin M. Dinges, William J. Cook, Patrick M. Schlievert, Douglas H. Ohlendorf

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21 Scopus citations

Abstract

The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 Å, 2.90 Å, and 2.75 Å, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry- related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1H at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, 1140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 Å resolution).

Original languageEnglish (US)
Pages (from-to)1220-1227
Number of pages8
JournalProtein Science
Volume6
Issue number6
DOIs
StatePublished - Jun 1997

Keywords

  • Protein conformation
  • Staphylococcus aureus
  • Superantigens
  • Toxins
  • X-ray crystallography

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