Reduced surface adsorption in 3D printed acrylonitrile butadiene styrene micro free-flow electrophoresis devices

Sarah K. Anciaux, Michael T. Bowser

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


We have 3D printed and fabricated micro free-flow electrophoresis (µFFE) devices in acrylonitrile butadiene styrene (ABS) that exhibit minimal surface adsorption without requiring additional surface coatings or specialized buffer additives. 2D, nano LC–micro free flow electrophoresis (2D nLC × µFFE) separations were used to assess both spatial and temporal broadening as peaks eluted through the separation channel. Minimal broadening due to wall adsorption was observed in either the spatial or temporal dimensions during separations of rhodamine 110, rhodamine 123, and fluorescein. Surface adsorption was observed in separations of Chromeo P503 labeled myoglobin and cytochrome c but was significantly reduced compared to previously reported glass devices. Peak widths of < 30 s were observed for both proteins. For comparison, Chromeo P503 labeled myoglobin and cytochrome c adsorb strongly to the surface of glass µFFE devices resulting in peak widths >20 min. A 2D nLC × µFFE separation of a Chromeo P503 labeled tryptic digest of BSA was performed to demonstrate the high peak capacity possible due to the low surface adsorption in the 3D printed ABS devices, even in the absence of surface coatings or buffer additives.

Original languageEnglish (US)
Pages (from-to)225-234
Number of pages10
Issue number3-4
StatePublished - Feb 1 2020

Bibliographical note

Funding Information:
The authors gratefully acknowledge funding from the National Science Foundation (CHE-1152022) and the National Institutes of Health (R01DK113091).

Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Copyright 2020 Elsevier B.V., All rights reserved.


  • 2D separations
  • 3D printing
  • ABS
  • micro free flow electrophoresis
  • surface adsorption

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

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