Reduced sarcoplasmic reticulum Ca2+ pump activity is antiarrhythmic in ischemic cardiomyopathy

Research output: Contribution to journalArticlepeer-review

Abstract

Background: We have described an arrhythmic mechanism seen only in cardiomyopathy that involves increased mitochondrial Ca2+ handling and selective transfer of Ca2+ to the sarcoplasmic reticulum (SR). Modeling suggested that mitochondrial Ca2+ transfer to the SR via type 2a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a) is a crucial element of this arrhythmic mechanism. Objective: We tested the role of SERCA2a in arrhythmias during ischemic cardiomyopathy. Methods: Myocardial infarction (MI) was induced in wild-type (Wt) and SERCA2a heterozygous knockdown (SERCA+/−) mice. Results: Compared with Wt MI mice, SERCA2a heterozygous knockdown (SERCA+/−) MI mice had a substantially lower mortality after 3 weeks of MI without a significant change in MI area. Aside from a significant delay of the cytoplasmic Ca2+ transient decay existed in SERCA+/− compared with Wt, SERCA+/− did not affect cardiac systolic and diastolic function at the whole organ or single cell levels either before or after MI. After MI, SERCA+/− mice had reduced SERCA2a expression in the MI border zone compared with Wt MI mice. SERCA+/− mice had significantly decreased corrected QT intervals and less ventricular tachycardia compared with Wt MI mice. SERCA+/− cardiomyocytes from MI mice showed a reduced action potential duration and reduced triggered activity compared with Wt MI cardiomyocytes. Reduction in arrhythmic risk was accompanied by reduced diastolic SR Ca2+ sparks, reduced SR Ca2+ content, reduced oxidized ryanodine receptor, and increased calsequestrin 2 in SERCA+/− MI mice. Conclusion: SERCA2a knockdown was antiarrhythmic after MI without affecting overall systolic performance. Possible antiarrhythmic mechanisms included reduced SR free Ca2+ and reduced diastolic SR Ca2+ release.

Original languageEnglish (US)
Pages (from-to)2107-2114
Number of pages8
JournalHeart Rhythm
Volume19
Issue number12
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
Funding Sources: This study was supported by the National Institutes of Health (R01 HL106592 and R01 HL104025, to Dr Dudley).

Publisher Copyright:
© 2022 Heart Rhythm Society

Keywords

  • Arrhythmia
  • Cardiac function
  • Heart failure
  • Myocardial infarction
  • SERCA2a

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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