Reduced prostaglandin synthesis by renal and aortic tissues from adult rats fed essential fatty acid-deficient diet after food deprivation

Hassan M. Sakr; Earl W Dunham

doi:10.1016/0262-1746(85)90018-6

Reduced prostaglandin synthesis by renal and aortic tissues from adult rats fed essential fatty acid-deficient diet after food deprivation

Hassan M. Sakr
, Earl W Dunham

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Studies were conducted to determine the efficacy of a dietary technique for reducing prostaglandin (PG) synthesis in adult rats. Rats weighing 280-318 g were fed either essential fatty acid (EFA)-deficient or EFA-adequate diets for 10-17 days after a period of food deprivation. Synthesis of renal papillary PGE 2 and aortic PGI2 from endogenous precursor in vitro were estimated by liquid chromatographic and bioassay/radioimmunoassay techniques, respectively, as indices of the capacity of the technique to induce EFA deficiency. PGE synthesis and PGI2 synthesis by isolated tissues from rats fed the EFA-deficient diet were significantly decreased (ca. 50%) relative to control rats fed an EFA-adequate diet. Body and renal papillary weights were not significantly altered by the EFA-deficient diet.

Original language	English (US)
Pages (from-to)	183-192
Number of pages	10
Journal	Prostaglandins Leukotrienes and Medicine
Volume	18
Issue number	2
DOIs	https://doi.org/10.1016/0262-1746(85)90018-6
State	Published - May 1985

Bibliographical note

Funding Information:
The authors thank Drs. J. Pike (The Upjohn Company) and M. Tsuboshima (Ono Pharmaceutical Co., LTD.) for generous gifts of prostanoids. This work was supported in part by NIH grants HL26111 and HL17871, and aid for Dr. Sakr’s studies was provided by the Egyptian government. We are also indebted to Patricia Maki for technical assistance, Dr. Ben G. Zirnmerman and Pamela Vandervort for the 6-keto-PGF1, antiserum and advice in the conduct of the RIA, and to Jackie Dokken for assistance in preparation of the manuscript.

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title = "Reduced prostaglandin synthesis by renal and aortic tissues from adult rats fed essential fatty acid-deficient diet after food deprivation",

abstract = "Studies were conducted to determine the efficacy of a dietary technique for reducing prostaglandin (PG) synthesis in adult rats. Rats weighing 280-318 g were fed either essential fatty acid (EFA)-deficient or EFA-adequate diets for 10-17 days after a period of food deprivation. Synthesis of renal papillary PGE 2 and aortic PGI2 from endogenous precursor in vitro were estimated by liquid chromatographic and bioassay/radioimmunoassay techniques, respectively, as indices of the capacity of the technique to induce EFA deficiency. PGE synthesis and PGI2 synthesis by isolated tissues from rats fed the EFA-deficient diet were significantly decreased (ca. 50\%) relative to control rats fed an EFA-adequate diet. Body and renal papillary weights were not significantly altered by the EFA-deficient diet.",

author = "Sakr, \{Hassan M.\} and Dunham, \{Earl W\}",

note = "Funding Information: The authors thank Drs. J. Pike (The Upjohn Company) and M. Tsuboshima (Ono Pharmaceutical Co., LTD.) for generous gifts of prostanoids. This work was supported in part by NIH grants HL26111 and HL17871, and aid for Dr. Sakr{\textquoteright}s studies was provided by the Egyptian government. We are also indebted to Patricia Maki for technical assistance, Dr. Ben G. Zirnmerman and Pamela Vandervort for the 6-keto-PGF1, antiserum and advice in the conduct of the RIA, and to Jackie Dokken for assistance in preparation of the manuscript.",

year = "1985",

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doi = "10.1016/0262-1746(85)90018-6",

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AU - Sakr, Hassan M.

AU - Dunham, Earl W

N1 - Funding Information: The authors thank Drs. J. Pike (The Upjohn Company) and M. Tsuboshima (Ono Pharmaceutical Co., LTD.) for generous gifts of prostanoids. This work was supported in part by NIH grants HL26111 and HL17871, and aid for Dr. Sakr’s studies was provided by the Egyptian government. We are also indebted to Patricia Maki for technical assistance, Dr. Ben G. Zirnmerman and Pamela Vandervort for the 6-keto-PGF1, antiserum and advice in the conduct of the RIA, and to Jackie Dokken for assistance in preparation of the manuscript.

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N2 - Studies were conducted to determine the efficacy of a dietary technique for reducing prostaglandin (PG) synthesis in adult rats. Rats weighing 280-318 g were fed either essential fatty acid (EFA)-deficient or EFA-adequate diets for 10-17 days after a period of food deprivation. Synthesis of renal papillary PGE 2 and aortic PGI2 from endogenous precursor in vitro were estimated by liquid chromatographic and bioassay/radioimmunoassay techniques, respectively, as indices of the capacity of the technique to induce EFA deficiency. PGE synthesis and PGI2 synthesis by isolated tissues from rats fed the EFA-deficient diet were significantly decreased (ca. 50%) relative to control rats fed an EFA-adequate diet. Body and renal papillary weights were not significantly altered by the EFA-deficient diet.

AB - Studies were conducted to determine the efficacy of a dietary technique for reducing prostaglandin (PG) synthesis in adult rats. Rats weighing 280-318 g were fed either essential fatty acid (EFA)-deficient or EFA-adequate diets for 10-17 days after a period of food deprivation. Synthesis of renal papillary PGE 2 and aortic PGI2 from endogenous precursor in vitro were estimated by liquid chromatographic and bioassay/radioimmunoassay techniques, respectively, as indices of the capacity of the technique to induce EFA deficiency. PGE synthesis and PGI2 synthesis by isolated tissues from rats fed the EFA-deficient diet were significantly decreased (ca. 50%) relative to control rats fed an EFA-adequate diet. Body and renal papillary weights were not significantly altered by the EFA-deficient diet.

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Reduced prostaglandin synthesis by renal and aortic tissues from adult rats fed essential fatty acid-deficient diet after food deprivation

Abstract

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