Reduced infectivity in cattle for an outer membrane protein mutant of Anaplasma marginale

Francy L. Crosby, Kelly A. Brayton, Forgivemore Magunda, Ulrike G Munderloh, Karen L. Kelley, Anthony F. Barbet

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Anaplasma marginale is the causative agent of anaplasmosis in cattle. Transposon mutagenesis of this pathogen using the Himar1 system resulted in the isolation of an omp10 operon insertional mutant referred to as the omp10::himar1 mutant. The work presented here evaluated if this mutant had morphological and/or growth rate defects compared to wild-type A. marginale. Results showed that the morphology, developmental cycle, and growth in tick and mammalian cell cultures are similar for the mutant and the wild type. Tick transmission experiments established that tick infection levels with the mutant were similar to those with wild-type A. marginale and that infected ticks successfully infected cattle. However, this mutant exhibited reduced infectivity and growth in cattle. The possibility of transforming A. marginale by transposon mutagenesis coupled with in vitro and in vivo assessment of altered phenotypes can aid in the identification of genes associated with virulence. The isolation of deliberately attenuated organisms that can be evaluated in their natural biological system is an important advance for the rational design of vaccines against this species.

Original languageEnglish (US)
Pages (from-to)2206-2214
Number of pages9
JournalApplied and Environmental Microbiology
Volume81
Issue number6
DOIs
StatePublished - Jan 1 2015

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Anaplasma marginale
outer membrane proteins
infectivity
Ticks
cattle
Membrane Proteins
pathogenicity
tick
membrane
mutants
protein
ticks
Mutagenesis
Growth
Anaplasmosis
mutagenesis
transposons
Operon
Virulence
anaplasmosis

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Reduced infectivity in cattle for an outer membrane protein mutant of Anaplasma marginale. / Crosby, Francy L.; Brayton, Kelly A.; Magunda, Forgivemore; Munderloh, Ulrike G; Kelley, Karen L.; Barbet, Anthony F.

In: Applied and Environmental Microbiology, Vol. 81, No. 6, 01.01.2015, p. 2206-2214.

Research output: Contribution to journalArticle

Crosby, Francy L. ; Brayton, Kelly A. ; Magunda, Forgivemore ; Munderloh, Ulrike G ; Kelley, Karen L. ; Barbet, Anthony F. / Reduced infectivity in cattle for an outer membrane protein mutant of Anaplasma marginale. In: Applied and Environmental Microbiology. 2015 ; Vol. 81, No. 6. pp. 2206-2214.
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