Recovery of human immunodeficiency virus type 1 from supernatant fluid of routine viral cultures

E. O. Sia, J. A. Englund, K. Sannerud, K. Hansen, N. Shah, Henry H Balfour

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral blood mononuclear cell samples from human immunodeficiency virus (HIV) antibody-positive subjects were inoculated into cell cultures used for routine viral isolation to determine if HIV could survive in them. HIV was recovered from 5 (19%) of 26 day 1 supernatants and from four of the five samples in all three types of cell cultures. We conclude that HIV may survive for at least 24 h in standard virology cell culture systems and therefore is a potential risk for laboratory personnel.

Original languageEnglish (US)
Pages (from-to)1664-1666
Number of pages3
JournalJournal of clinical microbiology
Volume27
Issue number7
DOIs
StatePublished - 1989

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