Recovery from the DNA replication checkpoint

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Checkpoint recovery is integral to a successful checkpoint response. Checkpoint pathways monitor progress during cell division so that in the event of an error, the checkpoint is activated to block the cell cycle and activate repair pathways. Intrinsic to this process is that once repair has been achieved, the checkpoint signaling pathway is inactivated and cell cycle progression resumes. We use the term “checkpoint recovery” to describe the pathways responsible for the inactivation of checkpoint signaling and cell cycle re-entry after the initial stress has been alleviated. The DNA replication or S-phase checkpoint monitors the integrity of DNA synthesis. When replication stress is encountered, replication forks are stalled, and the checkpoint signaling pathway is activated. Central to recovery from the S-phase checkpoint is the restart of stalled replication forks. If checkpoint recovery fails, stalled forks may become unstable and lead to DNA breaks or unusual DNA structures that are difficult to resolve, causing genomic instability. Alternatively, if cell cycle resumption mechanisms become uncoupled from checkpoint inactivation, cells with under-replicated DNA might proceed through the cell cycle, also diminishing genomic stability. In this review, we discuss the molecular mechanisms that contribute to inactivation of the S-phase checkpoint signaling pathway and the restart of replication forks during recovery from replication stress.

Original languageEnglish (US)
Article number94
JournalGenes
Volume7
Issue number11
DOIs
StatePublished - Nov 2016

Fingerprint

S Phase Cell Cycle Checkpoints
DNA Replication
Cell Cycle
Genomic Instability
DNA
DNA Breaks
Cell Cycle Checkpoints
Cell Division

Keywords

  • Checkpoint recovery
  • DNA replication
  • Fork restart
  • S-phase checkpoint

Cite this

Recovery from the DNA replication checkpoint. / Chaudhury, Indrajit; Koepp, Deanna M.

In: Genes, Vol. 7, No. 11, 94, 11.2016.

Research output: Contribution to journalReview article

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