Reconstruction of three-dimensional spatial patterns of rat cerebellar cortex activity in vivo using voltage sensitive dyes and optical sectioning

Haedeuk Yae, Stephen A. Elias, Timothy J Ebner

Research output: Contribution to journalConference article

3 Citations (Scopus)

Abstract

The three-dimensional neuronal activity of parallel fibers and Purkinje cells in rat cerebellar cortex is investigated in vivo. Using a voltage-sensitive dye (RH795) and imaging under an epi-fluorescence microscope and a CCD video camera, the response of cerebellar neurons to electrical stimulation of parallel fibers is studied. A deblurring algorithm is used on serial sectioned images to refine the spatial pattern of optical activity as well as to reveal the underlying neuronal events. This method reduces the error from out-of-focus components, improving the quantification and mapping of neuronal activity at low magnification.

Original languageEnglish (US)
Pages (from-to)538-539
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume11 pt 2
StatePublished - Dec 1 1989
EventImages of the Twenty-First Century - Proceedings of the 11th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 2 - Seattle, WA, USA
Duration: Nov 9 1989Nov 12 1989

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Voltage-Sensitive Dye Imaging
Optical Rotation
Cerebellar Cortex
Purkinje Cells
Electric Stimulation
Rats
Coloring Agents
Dyes
Fluorescence
Neurons
Fibers
Electric potential
Video cameras
CCD cameras
Microscopes
Imaging techniques
RH 795

Cite this

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abstract = "The three-dimensional neuronal activity of parallel fibers and Purkinje cells in rat cerebellar cortex is investigated in vivo. Using a voltage-sensitive dye (RH795) and imaging under an epi-fluorescence microscope and a CCD video camera, the response of cerebellar neurons to electrical stimulation of parallel fibers is studied. A deblurring algorithm is used on serial sectioned images to refine the spatial pattern of optical activity as well as to reveal the underlying neuronal events. This method reduces the error from out-of-focus components, improving the quantification and mapping of neuronal activity at low magnification.",
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T1 - Reconstruction of three-dimensional spatial patterns of rat cerebellar cortex activity in vivo using voltage sensitive dyes and optical sectioning

AU - Yae, Haedeuk

AU - Elias, Stephen A.

AU - Ebner, Timothy J

PY - 1989/12/1

Y1 - 1989/12/1

N2 - The three-dimensional neuronal activity of parallel fibers and Purkinje cells in rat cerebellar cortex is investigated in vivo. Using a voltage-sensitive dye (RH795) and imaging under an epi-fluorescence microscope and a CCD video camera, the response of cerebellar neurons to electrical stimulation of parallel fibers is studied. A deblurring algorithm is used on serial sectioned images to refine the spatial pattern of optical activity as well as to reveal the underlying neuronal events. This method reduces the error from out-of-focus components, improving the quantification and mapping of neuronal activity at low magnification.

AB - The three-dimensional neuronal activity of parallel fibers and Purkinje cells in rat cerebellar cortex is investigated in vivo. Using a voltage-sensitive dye (RH795) and imaging under an epi-fluorescence microscope and a CCD video camera, the response of cerebellar neurons to electrical stimulation of parallel fibers is studied. A deblurring algorithm is used on serial sectioned images to refine the spatial pattern of optical activity as well as to reveal the underlying neuronal events. This method reduces the error from out-of-focus components, improving the quantification and mapping of neuronal activity at low magnification.

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M3 - Conference article

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SP - 538

EP - 539

JO - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

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