The three-dimensional neuronal activity of parallel fibers and Purkinje cells in rat cerebellar cortex is investigated in vivo. Using a voltage-sensitive dye (RH795) and imaging under an epi-fluorescence microscope and a CCD video camera, the response of cerebellar neurons to electrical stimulation of parallel fibers is studied. A deblurring algorithm is used on serial sectioned images to refine the spatial pattern of optical activity as well as to reveal the underlying neuronal events. This method reduces the error from out-of-focus components, improving the quantification and mapping of neuronal activity at low magnification.
|Original language||English (US)|
|Number of pages||2|
|Journal||Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings|
|Volume||11 pt 2|
|State||Published - Dec 1 1989|
|Event||Images of the Twenty-First Century - Proceedings of the 11th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 2 - Seattle, WA, USA|
Duration: Nov 9 1989 → Nov 12 1989