Recombination between North American strains of porcine reproductive and respiratory syndrome virus

Shishan Yuan, Chris J. Nelsen, Michael P Murtaugh, Beverly J. Schmitt, Kay S. Faaberg

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV), a recently discovered arterivirus swine pathogen, was shown to undergo homologous recombination. Co-infection of MA-104 cells with two culture-adapted North American PRRSV strains resulted in recombinant viral particles containing chimeric ORF 3 and ORF 4 proteins. Nucleotide sequence analysis of cloned recombinant PCR products, encompassing 1182 bases of the 15.4 kb viral genome, revealed six independent recombination events. Recombinant products persisted in culture for at least three passages, indicating continuous formation of recombinant viruses, growth of recombinant viruses in competition with parental viruses, or both. The frequency of recombination was estimated from <2% up to 10% in the 1182 b fragment analyzed, which is similar to recombination frequencies observed in coronaviruses. An apparent example of natural ORF 5 recombination between naturally occurring wild type viruses was also found, indicating that recombination is likely an important genetic mechanism contributing to PRRSV evolution. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)87-98
Number of pages12
JournalVirus research
Volume61
Issue number1
DOIs
StatePublished - May 1 1999

Keywords

  • Differential RT-PCR
  • Porcine reproductive and respiratory syndrome virus
  • Viral recombination

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