Recombinant tri-segmented Pichinde virus as a novel live viral vaccine platform

Rekha Dhanwani, Hinh Ly, Yuying Liang

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages169-179
Number of pages11
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1581
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by National Institutes of HealthNIH grants R21AI094133 and R01AI083409 to Y.L., and R01AI093580 to H.L.

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

Keywords

  • Arenavirus
  • Pichinde virus
  • Vaccine
  • Viral vaccine vector

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