Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||11|
|State||Published - 2017|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
This work was supported by National Institutes of HealthNIH grants R21AI094133 and R01AI083409 to Y.L., and R01AI093580 to H.L.
© Springer Science+Business Media LLC 2017.
- Pichinde virus
- Viral vaccine vector