Abstract
Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.
Original language | English (US) |
---|---|
Pages (from-to) | 992-995 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 10 |
Issue number | 10 |
DOIs | |
State | Published - Oct 2013 |