Recombinant antibodies to histone post-translational modifications

Takamitsu Hattori; Joseph M. Taft; Kalina M. Swist; Hao Luo; Heather Witt; Matthew Slattery; Akiko Koide; Alexander J. Ruthenburg; Krzysztof Krajewski; Brian D. Strahl; Kevin P. White; Peggy J. Farnham; Yingming Zhao; Shohei Koide

doi:10.1038/nmeth.2605

Recombinant antibodies to histone post-translational modifications

Takamitsu Hattori, Joseph M. Taft, Kalina M. Swist, Hao Luo, Heather Witt, Matthew Slattery, Akiko Koide, Alexander J. Ruthenburg, Krzysztof Krajewski, Brian D. Strahl, Kevin P. White, Peggy J. Farnham, Yingming Zhao, Shohei Koide

Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.

Original language	English (US)
Pages (from-to)	992-995
Number of pages	4
Journal	Nature Methods
Volume	10
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.2605
State	Published - Oct 2013

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Recombinant antibodies to histone post-translational modifications. / Hattori, Takamitsu; Taft, Joseph M.; Swist, Kalina M.; Luo, Hao; Witt, Heather; Slattery, Matthew; Koide, Akiko; Ruthenburg, Alexander J.; Krajewski, Krzysztof; Strahl, Brian D.; White, Kevin P.; Farnham, Peggy J.; Zhao, Yingming; Koide, Shohei.

In: Nature Methods, Vol. 10, No. 10, 10.2013, p. 992-995.

Research output: Contribution to journal › Article › peer-review

Hattori, Takamitsu ; Taft, Joseph M. ; Swist, Kalina M. ; Luo, Hao ; Witt, Heather ; Slattery, Matthew ; Koide, Akiko ; Ruthenburg, Alexander J. ; Krajewski, Krzysztof ; Strahl, Brian D. ; White, Kevin P. ; Farnham, Peggy J. ; Zhao, Yingming ; Koide, Shohei. / Recombinant antibodies to histone post-translational modifications. In: Nature Methods. 2013 ; Vol. 10, No. 10. pp. 992-995.

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