Recognition of human recombinant Myelin Associated Glycoprotein by anti-carbohydrate antibodies of the L2/HNK-1 family

Mei Bigliardi-Qi, Guido C. Miescher, Andreas J. Steck

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The L2 and HNK-1 monoclonal antibodies recognize carbohydrate determinants containing sulfate-3-glucuronate that are prominent on cells of neural crest lineages. In humans these epitopes are most abundant on the Myelin Associated Glycoprotein and it was assumed that they co-localize on the same molecules. Recently, in vitro synthesized carbohydrates have provided a basis for the different recognition requirements of these two antibodies. We now provide in vivo evidence that a human melanoma cell line can produce glycoproteins such as fibronectin, which is recognized by both the L2 and HNK-1 antibodies, and simultaneously a transfected Myelin Associated Glycoprotein carrying only L2-type carbohydrates. Conceivably, the differential expression of L2-and HNK-1 type glycans could have a role in development.

Original languageEnglish (US)
Pages (from-to)171-178
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume217
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

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