Recent results and new hardware developments for protein crystal growth in microgravity

L. J. DeLucas, M. M. Long, K. M. Moore, W. M. Rosenblum, T. L. Bray, C. Smith, M. Carson, S. V.L. Narayana, M. D. Harrington, D. Carter, A. D. Clark, R. G. Nanni, J. Ding, A. Jacobo-Molina, G. Kamer, S. H. Hughes, E. Arnold, H. M. Einspahr, L. L. Clancy, G. S.J. RaoP. F. Cook, B. G. Harris, S. H. Munson, B. C. Finzel, A. McPherson, P. C. Weber, F. A. Lewandowski, T. L. Nagabhushan, P. P. Trotta, P. Reichert, M. A. Navia, K. P. Wilson, J. A. Thomson, R. N. Richards, K. D. Bowersox, C. J. Meade, E. S. Baker, S. P. Bishop, B. J. Dunbar, E. Trinh, J. Prahl, A. Sacco, C. E. Bugg

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Protein crystal growth experiments have been performed on 16 space shuttle missions since April 1985. The initial experiments used vapor diffusion crystallization techniques similar to those used in laboratories for earth-based experiments. More recent experiments have assessed temperature-induced crystallization as an alternative method for growing high quality protein crystals in microgravity. Results from both vapor-diffusion and temperature-induced crystallization experiments indicate that protein crystals grown in microgravity may be larger, display more uniform morphologies, and yield diffraction data to significantly higher resolutions than the best crystals of these proteins grown on earth.

Original languageEnglish (US)
Pages (from-to)183-195
Number of pages13
JournalJournal of Crystal Growth
Volume135
Issue number1-2
DOIs
StatePublished - Jan 1994

Bibliographical note

Funding Information:
This research was supported by NASA Con tract NAS8-36611 and NASA Grant NAGW-813. We would like to thank the engineers and technicians from the University of Alabama at Birmingham, Marshall Space Flight Center and Space Industries, Inc., and the space shuttle crews for their valuable participation in this project.

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