Recent progress in enzymatic protein labelling techniques and their applications

Yi Zhang, Keun Young Park, Kiall F. Suazo, Mark D Distefano

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

Protein-based conjugates are valuable constructs for a variety of applications. Conjugation of proteins to fluorophores is commonly used to study their cellular localization and the protein-protein interactions. Modification of therapeutic proteins with either polymers or cytotoxic moieties greatly enhances their pharmacokinetics or potency. To label a protein of interest, conventional direct chemical reaction with the side-chains of native amino acids often yields heterogeneously modified products. This renders their characterization complicated, requires difficult separation steps and may impact protein function. Although modification can also be achieved via the insertion of unnatural amino acids bearing bioorthogonal functional groups, these methods can have lower protein expression yields, limiting large scale production. As a site-specific modification method, enzymatic protein labelling is highly efficient and robust under mild reaction conditions. Significant progress has been made over the last five years in modifying proteins using enzymatic methods for numerous applications, including the creation of clinically relevant conjugates with polymers, cytotoxins or imaging agents, fluorescent or affinity probes to study complex protein interaction networks, and protein-linked materials for biosensing. This review summarizes developments in enzymatic protein labelling over the last five years for a panel of ten enzymes, including sortase A, subtiligase, microbial transglutaminase, farnesyltransferase, N-myristoyltransferase, phosphopantetheinyl transferases, tubulin tyrosin ligase, lipoic acid ligase, biotin ligase and formylglycine generating enzyme.

Original languageEnglish (US)
Pages (from-to)9106-9136
Number of pages31
JournalChemical Society Reviews
Volume47
Issue number24
DOIs
StatePublished - Dec 21 2018

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Labeling
Proteins
Ligases
Polymers
Bearings (structural)
Farnesyltranstransferase
Amino Acids
Thioctic Acid
Transglutaminases
Pharmacokinetics
Fluorophores
Cytotoxins
Enzymes
Tubulin
Biotin
Fluorescent Dyes
Functional groups
Labels
Chemical reactions
Imaging techniques

PubMed: MeSH publication types

  • Journal Article
  • Review

Cite this

Recent progress in enzymatic protein labelling techniques and their applications. / Zhang, Yi; Park, Keun Young; Suazo, Kiall F.; Distefano, Mark D.

In: Chemical Society Reviews, Vol. 47, No. 24, 21.12.2018, p. 9106-9136.

Research output: Contribution to journalReview article

Zhang, Yi ; Park, Keun Young ; Suazo, Kiall F. ; Distefano, Mark D. / Recent progress in enzymatic protein labelling techniques and their applications. In: Chemical Society Reviews. 2018 ; Vol. 47, No. 24. pp. 9106-9136.
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