Abstract
Methods employing isotope labeled compounds have been an important part of the bioanalytical canon for many decades. The past fifteen years have seen the development of many new approaches using stable (non-radioactive) isotopes as labels for high-throughput bioanalytical, ‘omics-scale’ measurements of metabolites (metabolomics) and proteins (proteomics). This review examines stable isotopic labeling approaches that have been developed for labeling whole intact plants, plant tissues, or crude extracts of plant materials with stable isotopes (mainly using 2H, 13C, 15N, 18O or 34S). The application of metabolome-scale labeling for improving metabolite annotation, metabolic pathway elucidation, and relative quantification in mass spectrometry-based metabolomics of plants is also reviewed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 41-48 |
| Number of pages | 8 |
| Journal | Current Opinion in Biotechnology |
| Volume | 43 |
| DOIs | |
| State | Published - Feb 1 2017 |
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Recent advances in stable isotope-enabled mass spectrometry-based plant metabolomics. / Freund, Dana M.; Hegeman, Adrian D.
In: Current Opinion in Biotechnology, Vol. 43, 01.02.2017, p. 41-48.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Recent advances in stable isotope-enabled mass spectrometry-based plant metabolomics
AU - Freund, Dana M.
AU - Hegeman, Adrian D.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Methods employing isotope labeled compounds have been an important part of the bioanalytical canon for many decades. The past fifteen years have seen the development of many new approaches using stable (non-radioactive) isotopes as labels for high-throughput bioanalytical, ‘omics-scale’ measurements of metabolites (metabolomics) and proteins (proteomics). This review examines stable isotopic labeling approaches that have been developed for labeling whole intact plants, plant tissues, or crude extracts of plant materials with stable isotopes (mainly using 2H, 13C, 15N, 18O or 34S). The application of metabolome-scale labeling for improving metabolite annotation, metabolic pathway elucidation, and relative quantification in mass spectrometry-based metabolomics of plants is also reviewed.
AB - Methods employing isotope labeled compounds have been an important part of the bioanalytical canon for many decades. The past fifteen years have seen the development of many new approaches using stable (non-radioactive) isotopes as labels for high-throughput bioanalytical, ‘omics-scale’ measurements of metabolites (metabolomics) and proteins (proteomics). This review examines stable isotopic labeling approaches that have been developed for labeling whole intact plants, plant tissues, or crude extracts of plant materials with stable isotopes (mainly using 2H, 13C, 15N, 18O or 34S). The application of metabolome-scale labeling for improving metabolite annotation, metabolic pathway elucidation, and relative quantification in mass spectrometry-based metabolomics of plants is also reviewed.
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UR - http://www.scopus.com/inward/citedby.url?scp=84985998179&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2016.08.002
DO - 10.1016/j.copbio.2016.08.002
M3 - Review article
VL - 43
SP - 41
EP - 48
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
SN - 0958-1669
ER -