Recent advances in stable isotope-enabled mass spectrometry-based plant metabolomics

Research output: Contribution to journalReview article

20 Citations (Scopus)

Abstract

Methods employing isotope labeled compounds have been an important part of the bioanalytical canon for many decades. The past fifteen years have seen the development of many new approaches using stable (non-radioactive) isotopes as labels for high-throughput bioanalytical, ‘omics-scale’ measurements of metabolites (metabolomics) and proteins (proteomics). This review examines stable isotopic labeling approaches that have been developed for labeling whole intact plants, plant tissues, or crude extracts of plant materials with stable isotopes (mainly using 2H, 13C, 15N, 18O or 34S). The application of metabolome-scale labeling for improving metabolite annotation, metabolic pathway elucidation, and relative quantification in mass spectrometry-based metabolomics of plants is also reviewed.

Original languageEnglish (US)
Pages (from-to)41-48
Number of pages8
JournalCurrent Opinion in Biotechnology
Volume43
DOIs
StatePublished - Feb 1 2017

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Metabolomics
Isotopes
Labeling
Mass spectrometry
Mass Spectrometry
Metabolites
Tissue Extracts
Metabolome
Plant Extracts
Metabolic Networks and Pathways
Complex Mixtures
Proteomics
Labels
Throughput
Tissue
Proteins

PubMed: MeSH publication types

  • Journal Article
  • Review

Cite this

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