Rearing the soil arthropod Folsomia candida (Collembola: Isotomidae) on agar plates and estimating biomass by protein staining with Ponceau S

Yang Grace Li; Ann M. Fallon

doi:10.1007/s13355-016-0405-8

Rearing the soil arthropod Folsomia candida (Collembola: Isotomidae) on agar plates and estimating biomass by protein staining with Ponceau S

Yang Grace Li, Ann M. Fallon

Entomology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Folsomia candida Willem (Collembola: Isotomidae) is a common soil arthropod used in standardized toxicological testing. We describe a simple method for rearing Collembola on agar plates and evaluating toxicity in the absence of charcoal, which has potential detoxifying effects. We developed a quantitative method for determining biomass by reversible protein staining of hexane-treated Collembola with Ponceau S. Protein staining has advantages over photometric methods for enumerating Collembola, and agar substrate provides a simple medium for testing toxicity of water-soluble substances independent of soil composition. Using these protocols, the 50 % effective concentration (EC₅₀) for the insecticide imidacloprid is ~0.2 µM, or 0.05 mg/kg agar substrate.

Original language	English (US)
Pages (from-to)	489-494
Number of pages	6
Journal	Applied Entomology and Zoology
Volume	51
Issue number	3
DOIs	https://doi.org/10.1007/s13355-016-0405-8
State	Published - Aug 1 2016

Bibliographical note

Funding Information:
This work was supported by the University of Minnesota Agricultural Experiment Station, St. Paul, MN. We appreciate helpful comments from anonymous reviewers. The authors have no conflicts to declare.

Publisher Copyright:
© 2016, The Japanese Society of Applied Entomology and Zoology.

Keywords

Charcoal
ISO standards
Imidacloprid
Spectrophotometry
Springtail

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10.1007/s13355-016-0405-8

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title = "Rearing the soil arthropod Folsomia candida (Collembola: Isotomidae) on agar plates and estimating biomass by protein staining with Ponceau S",

abstract = "Folsomia candida Willem (Collembola: Isotomidae) is a common soil arthropod used in standardized toxicological testing. We describe a simple method for rearing Collembola on agar plates and evaluating toxicity in the absence of charcoal, which has potential detoxifying effects. We developed a quantitative method for determining biomass by reversible protein staining of hexane-treated Collembola with Ponceau S. Protein staining has advantages over photometric methods for enumerating Collembola, and agar substrate provides a simple medium for testing toxicity of water-soluble substances independent of soil composition. Using these protocols, the 50 % effective concentration (EC50) for the insecticide imidacloprid is ~0.2 µM, or 0.05 mg/kg agar substrate.",

keywords = "Charcoal, ISO standards, Imidacloprid, Spectrophotometry, Springtail",

author = "Li, {Yang Grace} and Fallon, {Ann M.}",

note = "Funding Information: This work was supported by the University of Minnesota Agricultural Experiment Station, St. Paul, MN. We appreciate helpful comments from anonymous reviewers. The authors have no conflicts to declare. Publisher Copyright: {\textcopyright} 2016, The Japanese Society of Applied Entomology and Zoology.",

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T2 - Isotomidae) on agar plates and estimating biomass by protein staining with Ponceau S

AU - Li, Yang Grace

AU - Fallon, Ann M.

N1 - Funding Information: This work was supported by the University of Minnesota Agricultural Experiment Station, St. Paul, MN. We appreciate helpful comments from anonymous reviewers. The authors have no conflicts to declare. Publisher Copyright: © 2016, The Japanese Society of Applied Entomology and Zoology.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Folsomia candida Willem (Collembola: Isotomidae) is a common soil arthropod used in standardized toxicological testing. We describe a simple method for rearing Collembola on agar plates and evaluating toxicity in the absence of charcoal, which has potential detoxifying effects. We developed a quantitative method for determining biomass by reversible protein staining of hexane-treated Collembola with Ponceau S. Protein staining has advantages over photometric methods for enumerating Collembola, and agar substrate provides a simple medium for testing toxicity of water-soluble substances independent of soil composition. Using these protocols, the 50 % effective concentration (EC50) for the insecticide imidacloprid is ~0.2 µM, or 0.05 mg/kg agar substrate.

AB - Folsomia candida Willem (Collembola: Isotomidae) is a common soil arthropod used in standardized toxicological testing. We describe a simple method for rearing Collembola on agar plates and evaluating toxicity in the absence of charcoal, which has potential detoxifying effects. We developed a quantitative method for determining biomass by reversible protein staining of hexane-treated Collembola with Ponceau S. Protein staining has advantages over photometric methods for enumerating Collembola, and agar substrate provides a simple medium for testing toxicity of water-soluble substances independent of soil composition. Using these protocols, the 50 % effective concentration (EC50) for the insecticide imidacloprid is ~0.2 µM, or 0.05 mg/kg agar substrate.

KW - Charcoal

KW - ISO standards

KW - Imidacloprid

KW - Spectrophotometry

KW - Springtail

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ER -

Rearing the soil arthropod Folsomia candida (Collembola: Isotomidae) on agar plates and estimating biomass by protein staining with Ponceau S

Abstract

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