Rearing the soil arthropod Folsomia candida (Collembola: Isotomidae) on agar plates and estimating biomass by protein staining with Ponceau S

Yang Grace Li, Ann M. Fallon

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Folsomia candida Willem (Collembola: Isotomidae) is a common soil arthropod used in standardized toxicological testing. We describe a simple method for rearing Collembola on agar plates and evaluating toxicity in the absence of charcoal, which has potential detoxifying effects. We developed a quantitative method for determining biomass by reversible protein staining of hexane-treated Collembola with Ponceau S. Protein staining has advantages over photometric methods for enumerating Collembola, and agar substrate provides a simple medium for testing toxicity of water-soluble substances independent of soil composition. Using these protocols, the 50 % effective concentration (EC50) for the insecticide imidacloprid is ~0.2 µM, or 0.05 mg/kg agar substrate.

Original languageEnglish (US)
Pages (from-to)489-494
Number of pages6
JournalApplied Entomology and Zoology
Volume51
Issue number3
DOIs
StatePublished - Aug 1 2016

Keywords

  • Charcoal
  • ISO standards
  • Imidacloprid
  • Spectrophotometry
  • Springtail

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