The enhanced-sensitivity Trofile assay (TF-ES; Monogram Biosciences) was used to retest coreceptor tropism samples from 4 different cohorts of HIV-1-infected patients. Nine percent to 26% of patients with CCR5-tropic virus by the original Trofile assay had CXCR4-using virus by TF-ES. Lower CD4 cell counts were associated with CXCR4-using virus in all cohorts.
Bibliographical noteFunding Information:
Financial Support. The CPCRA and INSIGHT networks are funded by the National Institutes of Health (U01 AI-42170, U01 AI-46362, U01 AI-68641). The ACTG is funded by the National Institutes of Health (U01 AI-68636 and AI-68634 [Statistical and Data Analysis Center]). Other grants that funded coauthors include K23 AI55038 (to T.J.W.), K24 AI51966 (to R.M.G.), AI-69419 (Cornell Clinical Trials Unit), RR024996 (Cornell CTSC), and AI-46381 (Miriam Hospital Clinical Research Site). Monogram Biosciences received an SBIR grant from the National Institutes of Health (R44AI050321).