Real-Time Noninvasive Assessment of Pancreatic ATP Levels During Cold Preservation

W. E. Scott, S. Matsumoto, T. Tanaka, E. S. Avgoustiniatos, M. L. Graham, P. C. Williams, L. A. Tempelman, D. E. Sutherland, B. J. Hering, B. E. Hammer, K. K. Papas

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13 Scopus citations


31P-NMR spectroscopy was utilized to investigate rat and porcine pancreatic ATP:Pi ratios to assess the efficacy of existing protocols for cold preservation (CP) in maintaining organ quality. Following sacrifice, rat pancreata were immediately excised or left enclosed in the body for 15 minutes of warm ischemia (WI). After excision, rat pancreata were stored at 6°C to 8°C using histidine-tryptophan-ketoglutarate solution (HTK) presaturated with air (S1), HTK presaturated with O2 (S2), or the HTK/perfluorodecalin two-layer method (TLM) with both liquids presaturated with O2 (S3). 31P-NMR spectra were sequentially collected at 3, 6, 9, 12, and 24 hours of CP from pancreata stored with each of the three protocols examined. The ATP:Pi ratio for rat pancreata exposed to 15 minutes of WI and stored with S3 increased during the first 9 hours of CP, approaching values observed for organs procured with no WI. A marked reduction in the ATP:Pi ratio was observed beyond 12 hours of CP with S3. After 6 hours of CP, the ATP:Pi ratio was highest for S3, substantially decreased for S2, and below detection for S1. In sharp contrast to the rat model, ATP was barely detectable in porcine pancreata exposed to minimal warm ischemia (<15 minutes) stored with the TLM regardless of CP time. We conclude that 31P-NMR spectroscopy is a powerful tool that can be used to (1) noninvasively evaluate pancreata prior to islet isolation, (2) assess the efficacy of different preservation protocols, (3) precisely define the timing of reversible versus irreversible damage, and (4) assess whether intervention will extend this timing.

Original languageEnglish (US)
Pages (from-to)403-406
Number of pages4
JournalTransplantation proceedings
Issue number2
StatePublished - Mar 2008

Bibliographical note

Funding Information:
Research funding provided by grants from the National Center for Research Resources (U42 RR016598), National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (R43 DK070400), NIH, the Schott Foundation, and the Carol Olson Memorial Diabetes Research Fund.


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