Reactivation of rat peritoneal leukocyte and mast cell nuclei in heterokaryons with actively growing mouse cells

V. A. Polunovsky, M. E. Neverova, N. G. Khrushchov

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Leukocytes and mast cells of rat peritoneal exudate (PE) were fused in vitro with actively growing mouse cells. Segmented ring-shaped nuclei of granulocytes undergo drastic changes which result in dispersion of tightly condensed chromatin and gradual disappearance of the opening in the centre of the nucleus. These changes are paralleled by a resumption of RNA and DNA synthesis, as shown by autoradiography with [3H]uridine and [3H]thymidine. Solid inactive nuclei of mast cells, lymphocytes, monocytes and macrophages also resume DNA replication and high level of RNA synthesis. Fusion of thymidine kinase-deficient 3T3-4E cells with PE cells results in the incorporation of [3H]thymidine into the nuclei of heterokaryons. This may be considered evidence of the phenotypic expression of rat thymidine kinase gene in heterokaryons. A similar way in which segmented and non-segmented dormant nuclei undergo reactivation suggests that the reversibility of nuclear inactivation is a common feature of differentiated somatic cells.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
JournalCell Differentiation
Volume10
Issue number3
DOIs
StatePublished - May 1981

Keywords

  • cell fusion
  • reactivation of leukocytes
  • thymidine kinase

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