TY - JOUR
T1 - RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence
AU - Cekan, Pavol
AU - Hasegawa, Keisuke
AU - Pan, Yu
AU - Tubman, Emily
AU - Odde, David
AU - Chen, Jin Qiu
AU - Herrmann, Michelle A.
AU - Kumar, Sheetal
AU - Kalab, Petr
N1 - Publisher Copyright:
© 2016 Billmann, Horn, et al.
PY - 2016/4/15
Y1 - 2016/4/15
N2 - The coordination of cell cycle progression with the repair of DNA damage supports the genomic integrity of dividing cells. The function of many factors involved in DNA damage response (DDR) and the cell cycle depends on their Ran GTPase-regulated nuclear-cytoplasmic transport (NCT). The loading of Ran with GTP, which is mediated by RCC1, the guanine nucleotide exchange factor for Ran, is critical for NCT activity. However, the role of RCC1 or Ran•GTP in promoting cell proliferation or DDR is not clear. We show that RCC1 overexpression in normal cells increased cellular Ran•GTP levels and accelerated the cell cycle and DNA damage repair. As a result, normal cells overexpressing RCC1 evaded DNA damage-induced cell cycle arrest and senescence, mimicking colorectal carcinoma cells with high endogenous RCC1 levels. The RCC1-induced inhibition of senescence required Ran and exportin 1 and involved the activation of importin β-dependent nuclear import of 53BP1, a large NCT cargo. Our results indicate that changes in the activity of the Ran•GTP-regulated NCT modulate the rate of the cell cycle and the efficiency of DNA repair. Through the essential role of RCC1 in regulation of cellular Ran•GTP levels and NCT, RCC1 expression enables the proliferation of cells that sustain DNA damage.
AB - The coordination of cell cycle progression with the repair of DNA damage supports the genomic integrity of dividing cells. The function of many factors involved in DNA damage response (DDR) and the cell cycle depends on their Ran GTPase-regulated nuclear-cytoplasmic transport (NCT). The loading of Ran with GTP, which is mediated by RCC1, the guanine nucleotide exchange factor for Ran, is critical for NCT activity. However, the role of RCC1 or Ran•GTP in promoting cell proliferation or DDR is not clear. We show that RCC1 overexpression in normal cells increased cellular Ran•GTP levels and accelerated the cell cycle and DNA damage repair. As a result, normal cells overexpressing RCC1 evaded DNA damage-induced cell cycle arrest and senescence, mimicking colorectal carcinoma cells with high endogenous RCC1 levels. The RCC1-induced inhibition of senescence required Ran and exportin 1 and involved the activation of importin β-dependent nuclear import of 53BP1, a large NCT cargo. Our results indicate that changes in the activity of the Ran•GTP-regulated NCT modulate the rate of the cell cycle and the efficiency of DNA repair. Through the essential role of RCC1 in regulation of cellular Ran•GTP levels and NCT, RCC1 expression enables the proliferation of cells that sustain DNA damage.
UR - http://www.scopus.com/inward/record.url?scp=84963516806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963516806&partnerID=8YFLogxK
U2 - 10.1091/mbc.E16-01-0025
DO - 10.1091/mbc.E16-01-0025
M3 - Article
C2 - 26864624
AN - SCOPUS:84963516806
SN - 1059-1524
VL - 27
SP - 1346
EP - 1357
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 8
ER -