RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins

Antonia De Maio, Hari Krishna Yalamanchili, Carolyn J. Adamski, Vincenzo A. Gennarino, Zhandong Liu, Jun Qin, Sung Y. Jung, Ronald Richman, Harry T Orr, Huda Y. Zoghbi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

RNA splicing entails the coordinated interaction of more than 150 proteins in the spliceosome, one of the most complex of the cell's molecular machines. We previously discovered that the RNA-binding motif protein 17 (RBM17), a component of the spliceosome, is essential for survival and cell maintenance. Here, we find that it interacts with the spliceosomal factors U2SURP and CHERP and that they reciprocally regulate each other's stability, both in mouse and in human cells. Individual knockdown of each of the three proteins induces overlapping changes in splicing and gene expression of transcripts enriched for RNA-processing factors. Our results elucidate the function of RBM17, U2SURP, and CHERP and link the activity of the spliceosome to the regulation of downstream RNA-binding proteins. These data support the hypothesis that, beyond driving constitutive splicing, spliceosomal factors can regulate alternative splicing of specific targets. De Maio et al. find that the splicing factor RBM17 establishes a physical and functional relation with U2SURP and CHERP. Knockdown of these U2 snRNP-associated spliceosomal components reveals their synergistic activity toward regulation of a given set of transcripts rather than a more predictable transcriptome-wide inhibition of splicing.

Original languageEnglish (US)
Pages (from-to)726-736.e7
JournalCell Reports
Volume25
Issue number3
DOIs
StatePublished - Oct 16 2018

Fingerprint

RNA Splicing
RNA-Binding Proteins
Spliceosomes
RNA
Processing
Proteins
U2 Small Nuclear Ribonucleoproteins
Alternative Splicing
Transcriptome
Cell Survival
Gene expression
Maintenance
Gene Expression
Cells
RNA-Binding Motifs
Carrier Proteins
RNA Splicing Factors

Keywords

  • CHERP
  • RBM17
  • RNA homeostasis
  • U2SURP
  • cryptic splicing
  • spliceosome
  • splicing

PubMed: MeSH publication types

  • Journal Article

Cite this

De Maio, A., Yalamanchili, H. K., Adamski, C. J., Gennarino, V. A., Liu, Z., Qin, J., ... Zoghbi, H. Y. (2018). RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins. Cell Reports, 25(3), 726-736.e7. https://doi.org/10.1016/j.celrep.2018.09.041

RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins. / De Maio, Antonia; Yalamanchili, Hari Krishna; Adamski, Carolyn J.; Gennarino, Vincenzo A.; Liu, Zhandong; Qin, Jun; Jung, Sung Y.; Richman, Ronald; Orr, Harry T; Zoghbi, Huda Y.

In: Cell Reports, Vol. 25, No. 3, 16.10.2018, p. 726-736.e7.

Research output: Contribution to journalArticle

De Maio, A, Yalamanchili, HK, Adamski, CJ, Gennarino, VA, Liu, Z, Qin, J, Jung, SY, Richman, R, Orr, HT & Zoghbi, HY 2018, 'RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins', Cell Reports, vol. 25, no. 3, pp. 726-736.e7. https://doi.org/10.1016/j.celrep.2018.09.041
De Maio A, Yalamanchili HK, Adamski CJ, Gennarino VA, Liu Z, Qin J et al. RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins. Cell Reports. 2018 Oct 16;25(3):726-736.e7. https://doi.org/10.1016/j.celrep.2018.09.041
De Maio, Antonia ; Yalamanchili, Hari Krishna ; Adamski, Carolyn J. ; Gennarino, Vincenzo A. ; Liu, Zhandong ; Qin, Jun ; Jung, Sung Y. ; Richman, Ronald ; Orr, Harry T ; Zoghbi, Huda Y. / RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins. In: Cell Reports. 2018 ; Vol. 25, No. 3. pp. 726-736.e7.
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