Rational Design of Artificial Biofilms as Sustainable Supports for Whole-Cell Catalysis Through Integrating Extra- and Intracellular Catalysis

Hao Dong, Wenxue Zhang, Shengmin Zhou, Hanjie Ying, Ping Wang

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Biofilms are promising candidates for sustainable bioprocessing applications. This work presents a rational design of biofilm catalysts by integrating extra- and intracellular catalysis systems with optimized substrate channeling to realize efficient multistep biosynthesis. An assembly of four enzymes in a “three-in-one” structure was achieved by rationally placing the enzymes on curli nanofibers, the cell surface, and inside cells. The catalytic efficiency of the biofilm catalysts was over 2.8 folds higher than that of the control whole-cell catalysis when the substrate benzaldehyde was fed at 100 mm. The highest yield of d-phenyllactic acid catalyzed by biofilm catalysts under optimized conditions was 102.19 mm, also much higher than that of the control catalysis test (52.29 mm). The results demonstrate that engineered biofilms are greatly promising in integrating extra- and intracellular catalysis, illustrating great potentials of rational design in constructing biofilm catalysts as sustainable supports for whole-cell catalysis.

Original languageEnglish (US)
Article numbere202200850
JournalChemSusChem
Volume15
Issue number17
DOIs
StatePublished - Sep 7 2022

Bibliographical note

Funding Information:
This work was sponsored by the National Natural Science Foundation of China (21636003, 21672065, and 22077032), the International S&T Innovation Cooperation Key Project (2017YFE0129600), the Fundamental Research Funds for the Central Universities (22221818014), and the 111 Project (B18022).

Publisher Copyright:
© 2022 The Authors. ChemSusChem published by Wiley-VCH GmbH.

Keywords

  • biofilm catalysts
  • biotransformations
  • enzymes
  • immobilization
  • rational design

Fingerprint

Dive into the research topics of 'Rational Design of Artificial Biofilms as Sustainable Supports for Whole-Cell Catalysis Through Integrating Extra- and Intracellular Catalysis'. Together they form a unique fingerprint.

Cite this