Rational cytokine design for increased lifetime and enhanced potency using pH-activated “histidine switching”

Casim A. Sarkar, Ky Lowenhaupt, Thomas Horan, Thomas C. Boone, Bruce Tidor, Douglas A. Lauffenburger

Research output: Contribution to journalArticle

117 Scopus citations

Abstract

We describe a method for the rational design of more effective therapeutic proteins using amino acid substitutions that reduce receptor binding affinity in intracellular endosomal compartments, thereby leading to increased recycling in the ligand-sorting process and consequently resulting in longer half-life in extracellular medium. We demonstrate this approach for granulocyte colony-stimulating factor by using computationally predicted histidine substitutions that switch protonation states between cell-surface and endosomal pH. Molecular modeling of binding electrostatics indicates two different single-histidine mutants that fulfill our design requirements; experimental assays demonstrate that each mutant indeed exhibits an order-of-magnitude increase in medium half-life along with enhanced potency due to increased endocytic recycling.

Original languageEnglish (US)
Pages (from-to)908-913
Number of pages6
JournalNature biotechnology
Volume20
Issue number9
DOIs
StatePublished - Sep 2002

Fingerprint Dive into the research topics of 'Rational cytokine design for increased lifetime and enhanced potency using pH-activated “histidine switching”'. Together they form a unique fingerprint.

  • Cite this