Rates and controls of nitrification in a large oligotrophic lake

Gaston E. Small, George S. Bullerjahn, Robert W Sterner, Benjamin F.N. Beall, Sandra Brovold, Jacques C Finlay, Robert M.L. McKay, Maitreyee Mukherjee

Research output: Contribution to journalArticle

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Abstract

Recent discoveries have altered prevailing paradigms concerning the conditions under which nitrification takes place and the organisms responsible for nitrification in aquatic ecosystems. In Lake Superior, nitrate (NO-3) concentrations have increased fivefold in the past century. Although previous evidence indicated that most NO-3 is generated by nitrification within the lake, important questions remain concerning the magnitude and controls of nitrification, and which microbial groups are primarily responsible for this process. We measured water-column nitrification rates in the western basin of Lake Superior during five research cruises from November 2009 to March 2011. Using in situ bottle incubations at 10 depths, we quantified nitrification rates using both the oxidation of 15N-labeled ammonium (NH+4) and the uptake of 14C associated with nitrification. Average rates of NH+4 oxidation ranged from 18-34 nmol N L-1 d-1 across the five cruises, similar to values reported for the coastal ocean, and two orders of magnitude lower than values reported from other lakes. Low nitrification rates observed in the epilimnion corresponded to the absence of ammonium-oxidizing archaea and nitrite-oxidizing bacteria. The measured rates of nitrification are > 50-fold greater than the long-term NO-3 rise in the lake, indicating that N is actively cycling and that long-term change in this ecosystem is mediated by internal dynamics.

Original languageEnglish (US)
Pages (from-to)276-286
Number of pages11
JournalLimnology and Oceanography
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2013

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nitrification
lakes
lake
Lake Superior
ammonium
oxidation
rate
epilimnion
Archaea
long-term change
bottles
aquatic ecosystem
nitrites
nitrite
water column
oceans
incubation
nitrates
basins
nitrate

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Small, G. E., Bullerjahn, G. S., Sterner, R. W., Beall, B. F. N., Brovold, S., Finlay, J. C., ... Mukherjee, M. (2013). Rates and controls of nitrification in a large oligotrophic lake. Limnology and Oceanography, 58(1), 276-286. https://doi.org/10.4319/lo.2013.58.1.0276

Rates and controls of nitrification in a large oligotrophic lake. / Small, Gaston E.; Bullerjahn, George S.; Sterner, Robert W; Beall, Benjamin F.N.; Brovold, Sandra; Finlay, Jacques C; McKay, Robert M.L.; Mukherjee, Maitreyee.

In: Limnology and Oceanography, Vol. 58, No. 1, 01.01.2013, p. 276-286.

Research output: Contribution to journalArticle

Small, GE, Bullerjahn, GS, Sterner, RW, Beall, BFN, Brovold, S, Finlay, JC, McKay, RML & Mukherjee, M 2013, 'Rates and controls of nitrification in a large oligotrophic lake', Limnology and Oceanography, vol. 58, no. 1, pp. 276-286. https://doi.org/10.4319/lo.2013.58.1.0276
Small, Gaston E. ; Bullerjahn, George S. ; Sterner, Robert W ; Beall, Benjamin F.N. ; Brovold, Sandra ; Finlay, Jacques C ; McKay, Robert M.L. ; Mukherjee, Maitreyee. / Rates and controls of nitrification in a large oligotrophic lake. In: Limnology and Oceanography. 2013 ; Vol. 58, No. 1. pp. 276-286.
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