Ratchet nanofiltration of DNA

Joel D P Thomas, Mark N. Joswiak, Daniel W. Olson, Sung Gyu Park, Kevin D. Dorfman

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The DNA nanofilter is a microfabricated electrophoretic separation device consisting of a periodic array of thin slits (circa 60 nm) separated by deeper wells (circa 320 nm). We demonstrate that this device can act as a tuneable, clog-free filter when operating in a low frequency, asymmetric field inversion mode. This filtration occurs by using asymmetric field inversion to achieve bi-directional migration of short (less than 1000 bp) DNA. Moreover, similar ratchet-type operation can improve separations when compared to a constant field separation in the same device. These modes of operation enhance the utility of the DNA nanofilter as a component of integrated lab-on-a-chip devices. The experimental data confirm theoretical predictions for the bidirectional transport of DNA in entropy-based separations.

Original languageEnglish (US)
Pages (from-to)3741-3746
Number of pages6
JournalLab on a chip
Volume13
Issue number18
DOIs
StatePublished - Sep 21 2013

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