Ratchet nanofiltration of DNA

J. D P Thomas, D. W. Olson, M. N. Joswiak, S. G. Park, K. D. Dorfman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have created a non-clogging double stranded DNA filter using the nanofilter array DNA separation geometry. By applying pulsed electric fields in opposite directions we created a temporally asymmetric ratchet, which resulted in bidirectional migration of small DNA, less than 1000 base pairs. The direction of the DNA migration depends on the size of the DNA and can be tuned by changing the electric field strength and pulse length. It can be tuned such that only DNA below a desired size is allowed to pass though the device while the remaining DNA travels back towards the injection.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1212-1214
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • DNA separation
  • Microfluidics
  • Nanofilter array
  • Ratchet

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  • Cite this

    Thomas, J. D. P., Olson, D. W., Joswiak, M. N., Park, S. G., & Dorfman, K. D. (2013). Ratchet nanofiltration of DNA. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1212-1214). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.