Rat subcutaneous tissue response to calcium silicate containing different arsenic concentrations

Paloma Gagliardi Minotti, Ronald Ordinola-Zapata, Raquel Zanin Midena, Marina Angélica Marciano, Bruno Cavalini Cavenago, Clovis Monteiro Bramante, Roberto Brandão Garcia, Marco Antonio Hungaro Duarte, Ivaldo Gomes de Moraes

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Objective: To evaluate the response of rat subcutaneous tissue in implanted polyethylene tubes that were filled with GMTA Angelus and Portland cements containing different arsenic concentrations. Material and Methods: Atomic absorption spectrophotometry was utilized to obtain the values of the arsenic concentration in the materials. Thirty-six rats were divided into 3 groups of 12 animals for each experimental period. Each animal received two implants of polyethylene tubes filled with different test cements and the lateral of the tubes was used as a control group. After 15, 30 and 60 days of implantation, the animals were killed and the specimens were prepared for descrip0tive and morphometric analysis considering: inflammatory cells, collagen fibers, fibroblasts, blood vessels and other components. The results were analyzed utilizing the Kuskal-Wallis test and the Dunn's Multiple test for comparison (p<0.05). Results: The materials showed, according to atomic absorption spectrophotometry, the following doses of arsenic: GMTA Angelus: 5.01 mg/kg, WPC Irajazinho: 0.69 mg/kg, GPC Minetti: 18.46 mg/kg and GPC Votoran: 10.76 mg/kg. In a 60-day periods, all specimens displayed a neoformation of connective tissue with a structure of fibrocellular aspect (capsule). Control groups and MTA Angelus produced the lower amount of inflammatory reaction and GPC Minetti, the highest reaction. Conclusions: There was no direct relationship between the concentration of arsenic present in the composition of the materials and the intensity of the inflammatory reactions. Higher values, as 18.46 mg/kg of arsenic in the cement, produce characteristics of severe inflammation reaction at the 60-day period. The best results were found in MTA angelus.

Original languageEnglish (US)
Pages (from-to)42-48
Number of pages7
JournalJournal of Applied Oral Science
Issue number1
StatePublished - 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015, J Appl Oral Sci. All Rights Reserved.


  • Arsenic
  • Dental materials
  • Endodontics
  • Portland cement
  • Subcutaneous tissue reaction


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