RAS internal tandem duplication disrupts GTPase-activating protein (GAP) binding to activate oncogenic signaling

Andrew C Nelson, Thomas J Turbyville, Srisathiyanarayanan Dharmaiah, Megan Rigby, Rendong Yang, Ting-You Wang, John Columbus, Robert Stephens, Troy Taylor, Drew Sciacca, Getiria Onsongo, Anne Sarver, Subbaya Subramanian, Dwight V Nissley, Dhirendra K Simanshu, Emil Lou

Research output: Contribution to journalArticle

Abstract

The oncogene RAS is one of the most widely studied proteins in cancer biology, and mutant active RAS is a driver in many types of solid tumors and hematological malignancies. Yet the biological effects of different RAS mutations and the tissue-specific clinical implications are complex and nuanced. Here, we identified an internal tandem duplication (ITD) in the switch II domain of NRAS from a patient with extremely aggressive colorectal carcinoma. Results of whole-exome DNA sequencing of primary and metastatic tumors indicated that this mutation was present in all analyzed metastases and excluded the presence of any other clear oncogenic driver mutations. Biochemical analysis revealed increased interaction of the RAS ITD with Raf proto-oncogene Ser/Thr kinase (RAF), leading to increased phosphorylation of downstream MAPK/ERK kinase (MEK)/extracellular signal-regulated kinase (ERK). The ITD prevented interaction with neurofibromin 1 (NF1)-GTPase-activating protein (GAP), providing a mechanism for sustained activity of the RAS ITD protein. We present the first crystal structures of NRAS and KRAS ITD at 1.65-1.75 Å resolution, respectively, providing insight into the physical interactions of this class of RAS variants with its regulatory and effector proteins. Our in-depth bedside-to-bench analysis uncovers the molecular mechanism underlying a case of highly aggressive colorectal cancer and illustrates the importance of robust biochemical and biophysical approaches in the implementation of individualized medicine.

Original languageEnglish (US)
Pages (from-to)9335-9348
Number of pages14
JournalThe Journal of biological chemistry
Volume295
Issue number28
DOIs
StatePublished - Jul 10 2020

Keywords

  • bedside-to-bench analysis
  • colon cancer
  • exome sequencing
  • oncogene
  • personalized medicine
  • protein structure
  • RAS protein
  • structural biology
  • switch II domain
  • tandem duplication

PubMed: MeSH publication types

  • Journal Article

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  • Cite this

    Nelson, A. C., Turbyville, T. J., Dharmaiah, S., Rigby, M., Yang, R., Wang, T-Y., Columbus, J., Stephens, R., Taylor, T., Sciacca, D., Onsongo, G., Sarver, A., Subramanian, S., Nissley, D. V., Simanshu, D. K., & Lou, E. (2020). RAS internal tandem duplication disrupts GTPase-activating protein (GAP) binding to activate oncogenic signaling. The Journal of biological chemistry, 295(28), 9335-9348. https://doi.org/10.1074/jbc.RA119.011080