Rare genetic variation at Zea mays crtRB1 increases Β-carotene in maize grain

Jianbing Yan; Catherine Bermudez Kandianis; Carlos E. Harjes; Ling Bai; Eun Ha Kim; Xiaohong Yang; Debra J. Skinner; Zhiyuan Fu; Sharon Mitchell; Qing Li; Maria G.Salas Fernandez; Maria Zaharieva; Raman Babu; Yang Fu; Natalia Palacios; Jiansheng Li; Dean Dellapenna; Thomas Brutnell; Edward S. Buckler; Marilyn L. Warburton; Torbert Rocheford

doi:10.1038/ng.551

Rare genetic variation at Zea mays crtRB1 increases Β-carotene in maize grain

Jianbing Yan, Catherine Bermudez Kandianis, Carlos E. Harjes, Ling Bai, Eun Ha Kim, Xiaohong Yang, Debra J. Skinner, Zhiyuan Fu, Sharon Mitchell, Qing Li, Maria G.Salas Fernandez, Maria Zaharieva, Raman Babu, Yang Fu, Natalia Palacios, Jiansheng Li, Dean Dellapenna, Thomas Brutnell, Edward S. Buckler, Marilyn L. WarburtonTorbert Rocheford

Plant and Microbial Biology

Research output: Contribution to journal › Article › peer-review

429 Scopus citations

Abstract

Breeding to increase Β-carotene levels in cereal grains, termed provitamin A biofortification, is an economical approach to address dietary vitamin A deficiency in the developing world. Experimental evidence from association and linkage populations in maize (Zea mays L.) demonstrate that the gene encoding Β-carotene hydroxylase 1 (crtRB1) underlies a principal quantitative trait locus associated with Β-carotene concentration and conversion in maize kernels. crtRB1 alleles associated with reduced transcript expression correlate with higher Β-carotene concentrations. Genetic variation at crtRB1 also affects hydroxylation efficiency among encoded allozymes, as observed by resultant carotenoid profiles in recombinant expression assays. The most favorable crtRB1 alleles, rare in frequency and unique to temperate germplasm, are being introgressed via inexpensive PCR marker-assisted selection into tropical maize germplasm adapted to developing countries, where it is most needed for human health.

Original language	English (US)
Pages (from-to)	322-327
Number of pages	6
Journal	Nature Genetics
Volume	42
Issue number	4
DOIs	https://doi.org/10.1038/ng.551
State	Published - Apr 2010

Bibliographical note

Funding Information:
We thank K. Pixley and J. Chandler for seed and field experiment coordination and W. White, P. Bermudez, S. Islam, C. Paul, W. Liu, S. Xu and Y. Zhou for carotenoid HPLC profiling. Helpful comments on the manuscript from J. Wilkinson and J. Yu are appreciated. This research was supported by the US Agency for International Development (T.R.), HarvestPlus (T.R., D.D.P., J.Y., J.L., M.L.W.), National Science Foundation (NSF) DBI-0321467, DBI-0820619 (E.S.B.), NSF DBI-0604923 (T.R.), USDA-ARS (E.S.B.), NSF DBI-0501713 (T.B.), TRIAD Foundation (T.B.), China NSF-30821140352 (J.L., J.Y.), China Scholarship Fund (Y.F.) and JBT Fellowship-UIUC (C.B.K.).

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Yan, J., Kandianis, C. B., Harjes, C. E., Bai, L., Kim, E. H., Yang, X., Skinner, D. J., Fu, Z., Mitchell, S., Li, Q., Fernandez, M. G. S., Zaharieva, M., Babu, R., Fu, Y., Palacios, N., Li, J., Dellapenna, D., Brutnell, T., Buckler, E. S., ... Rocheford, T. (2010). Rare genetic variation at Zea mays crtRB1 increases Β-carotene in maize grain. Nature Genetics, 42(4), 322-327. https://doi.org/10.1038/ng.551

Yan, J, Kandianis, CB, Harjes, CE, Bai, L, Kim, EH, Yang, X, Skinner, DJ, Fu, Z, Mitchell, S, Li, Q, Fernandez, MGS, Zaharieva, M, Babu, R, Fu, Y, Palacios, N, Li, J, Dellapenna, D, Brutnell, T, Buckler, ES, Warburton, ML & Rocheford, T 2010, 'Rare genetic variation at Zea mays crtRB1 increases Β-carotene in maize grain', Nature Genetics, vol. 42, no. 4, pp. 322-327. https://doi.org/10.1038/ng.551

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title = "Rare genetic variation at Zea mays crtRB1 increases Β-carotene in maize grain",

abstract = "Breeding to increase Β-carotene levels in cereal grains, termed provitamin A biofortification, is an economical approach to address dietary vitamin A deficiency in the developing world. Experimental evidence from association and linkage populations in maize (Zea mays L.) demonstrate that the gene encoding Β-carotene hydroxylase 1 (crtRB1) underlies a principal quantitative trait locus associated with Β-carotene concentration and conversion in maize kernels. crtRB1 alleles associated with reduced transcript expression correlate with higher Β-carotene concentrations. Genetic variation at crtRB1 also affects hydroxylation efficiency among encoded allozymes, as observed by resultant carotenoid profiles in recombinant expression assays. The most favorable crtRB1 alleles, rare in frequency and unique to temperate germplasm, are being introgressed via inexpensive PCR marker-assisted selection into tropical maize germplasm adapted to developing countries, where it is most needed for human health.",

author = "Jianbing Yan and Kandianis, {Catherine Bermudez} and Harjes, {Carlos E.} and Ling Bai and Kim, {Eun Ha} and Xiaohong Yang and Skinner, {Debra J.} and Zhiyuan Fu and Sharon Mitchell and Qing Li and Fernandez, {Maria G.Salas} and Maria Zaharieva and Raman Babu and Yang Fu and Natalia Palacios and Jiansheng Li and Dean Dellapenna and Thomas Brutnell and Buckler, {Edward S.} and Warburton, {Marilyn L.} and Torbert Rocheford",

note = "Funding Information: We thank K. Pixley and J. Chandler for seed and field experiment coordination and W. White, P. Bermudez, S. Islam, C. Paul, W. Liu, S. Xu and Y. Zhou for carotenoid HPLC profiling. Helpful comments on the manuscript from J. Wilkinson and J. Yu are appreciated. This research was supported by the US Agency for International Development (T.R.), HarvestPlus (T.R., D.D.P., J.Y., J.L., M.L.W.), National Science Foundation (NSF) DBI-0321467, DBI-0820619 (E.S.B.), NSF DBI-0604923 (T.R.), USDA-ARS (E.S.B.), NSF DBI-0501713 (T.B.), TRIAD Foundation (T.B.), China NSF-30821140352 (J.L., J.Y.), China Scholarship Fund (Y.F.) and JBT Fellowship-UIUC (C.B.K.).",

year = "2010",

month = apr,

doi = "10.1038/ng.551",

language = "English (US)",

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pages = "322--327",

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T1 - Rare genetic variation at Zea mays crtRB1 increases Β-carotene in maize grain

AU - Yan, Jianbing

AU - Kandianis, Catherine Bermudez

AU - Harjes, Carlos E.

AU - Bai, Ling

AU - Kim, Eun Ha

AU - Yang, Xiaohong

AU - Skinner, Debra J.

AU - Fu, Zhiyuan

AU - Mitchell, Sharon

AU - Li, Qing

AU - Fernandez, Maria G.Salas

AU - Zaharieva, Maria

AU - Babu, Raman

AU - Fu, Yang

AU - Palacios, Natalia

AU - Li, Jiansheng

AU - Dellapenna, Dean

AU - Brutnell, Thomas

AU - Buckler, Edward S.

AU - Warburton, Marilyn L.

AU - Rocheford, Torbert

N1 - Funding Information: We thank K. Pixley and J. Chandler for seed and field experiment coordination and W. White, P. Bermudez, S. Islam, C. Paul, W. Liu, S. Xu and Y. Zhou for carotenoid HPLC profiling. Helpful comments on the manuscript from J. Wilkinson and J. Yu are appreciated. This research was supported by the US Agency for International Development (T.R.), HarvestPlus (T.R., D.D.P., J.Y., J.L., M.L.W.), National Science Foundation (NSF) DBI-0321467, DBI-0820619 (E.S.B.), NSF DBI-0604923 (T.R.), USDA-ARS (E.S.B.), NSF DBI-0501713 (T.B.), TRIAD Foundation (T.B.), China NSF-30821140352 (J.L., J.Y.), China Scholarship Fund (Y.F.) and JBT Fellowship-UIUC (C.B.K.).

PY - 2010/4

Y1 - 2010/4

N2 - Breeding to increase Β-carotene levels in cereal grains, termed provitamin A biofortification, is an economical approach to address dietary vitamin A deficiency in the developing world. Experimental evidence from association and linkage populations in maize (Zea mays L.) demonstrate that the gene encoding Β-carotene hydroxylase 1 (crtRB1) underlies a principal quantitative trait locus associated with Β-carotene concentration and conversion in maize kernels. crtRB1 alleles associated with reduced transcript expression correlate with higher Β-carotene concentrations. Genetic variation at crtRB1 also affects hydroxylation efficiency among encoded allozymes, as observed by resultant carotenoid profiles in recombinant expression assays. The most favorable crtRB1 alleles, rare in frequency and unique to temperate germplasm, are being introgressed via inexpensive PCR marker-assisted selection into tropical maize germplasm adapted to developing countries, where it is most needed for human health.

AB - Breeding to increase Β-carotene levels in cereal grains, termed provitamin A biofortification, is an economical approach to address dietary vitamin A deficiency in the developing world. Experimental evidence from association and linkage populations in maize (Zea mays L.) demonstrate that the gene encoding Β-carotene hydroxylase 1 (crtRB1) underlies a principal quantitative trait locus associated with Β-carotene concentration and conversion in maize kernels. crtRB1 alleles associated with reduced transcript expression correlate with higher Β-carotene concentrations. Genetic variation at crtRB1 also affects hydroxylation efficiency among encoded allozymes, as observed by resultant carotenoid profiles in recombinant expression assays. The most favorable crtRB1 alleles, rare in frequency and unique to temperate germplasm, are being introgressed via inexpensive PCR marker-assisted selection into tropical maize germplasm adapted to developing countries, where it is most needed for human health.

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JF - Nature Genetics

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ER -

Rare genetic variation at Zea mays crtRB1 increases Β-carotene in maize grain

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